Pig adiponectin,ADP ELISA Kit

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中文名称：

猪脂联素(ADP)酶联免疫试剂盒
货号：

CSB-E06839p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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引用文献

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪脂联素（ADP）酶联免疫检测试剂盒（货号CSB-E06839p），采用竞争性定量检测原理，适用于科研领域对猪血清、血浆及组织匀浆样本中ADP含量的精准分析。脂联素作为脂肪细胞分泌的重要激素，在调节糖脂代谢、胰岛素敏感性及能量平衡中发挥关键作用，其水平变化常与代谢性疾病研究相关。本试剂盒检测范围覆盖1.875-30 μg/mL，通过预包被特异性抗体与样本/标准品中的ADP竞争结合酶标抗原，经显色反应实现浓度测定，操作流程包含样本处理、孵育、洗涤及读数步骤。该试剂盒特别适用于动物实验中对猪模型代谢机制、营养干预效果或肥胖相关病理的基础研究，可支持不同生物样本中ADP的动态监测。产品包含标准品、酶结合物、显色底物等完整组分，提供96孔板规格，实验全程需自备酶标仪及移液设备，建议通过标准曲线拟合实现样本定量分析。本产品严格验证抗体特异性及批次稳定性，确保科研数据的可靠性和重复性，为畜牧营养学、比较医学等领域的分子机制探索提供有效工具。
缩写：

ADP
Uniprot No.：

Q6PP07
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.875 μg/mL-30 μg/mL
灵敏度：

1.875 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-94
1:2	Average %	96
	Range %	92-100
1:4	Average %	83
	Range %	80-86
1:8	Average %	91
	Range %	83-98

回收率：

The recovery of pig ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-99
EDTA plasma (n=4)	93	91-96