Goat adiponectin(ADP)ELISA Kit

产品详情

产品描述：

CUSABIO山羊脂联素（ADP）酶联免疫检测试剂盒（货号：CSB-E15981G），采用竞争法定量原理，可精准检测血清、血浆及组织匀浆样本中的ADP含量，检测灵敏度范围为0.312 ng/mL至20 ng/mL。脂联素作为脂肪细胞分泌的重要激素，在调节糖脂代谢平衡、增强胰岛素敏感性及抗炎过程中发挥关键作用，其水平变化常与代谢性疾病相关。本试剂盒通过样本中的ADP与酶标抗原竞争结合固相抗体，经显色反应后，吸光度值与ADP浓度呈负相关，结合标准曲线即可实现定量分析。该产品适用于肥胖、2型糖尿病、心血管疾病等代谢相关机制的基础研究，可支持动物模型或体外实验中脂联素表达水平的动态监测，为探索能量代谢调控、脂肪因子互作等科研方向提供可靠工具。试剂盒包含预包被板、标准品、检测抗体及全套实验缓冲液，操作流程优化，兼容常规实验室设备，满足多样本类型的高通量检测需求。
缩写：

ADP
Uniprot No.：

U6A087
种属：

Capra hircus (Goat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	95
	Range %	89-100
1:2000	Average %	92
	Range %	87-96
1:4000	Average %	99
	Range %	93-104
1:8000	Average %	104
	Range %	97-109

回收率：

The recovery of goat ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-97
EDTA plasma (n=4)	94	87-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.