Goat Luteinizing Hormone (LH) ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO山羊黄体生成素（Luteinizing Hormone, LH）酶联免疫检测试剂盒（货号：CSB-E13274G），基于竞争法原理实现定量分析，适用于血清、血浆及组织匀浆样本中LH含量的精准测定，检测灵敏度达0.3 mLU/mL，线性范围为0.3-60 mLU/mL。LH作为垂体前叶分泌的关键促性腺激素，在山羊生殖调控中发挥重要作用，可促进卵泡成熟、触发排卵及维持黄体功能，其水平变化与发情周期、繁殖性能及内分泌平衡密切相关。本试剂盒通过特异性抗体与样本中LH的竞争性结合反应，结合酶标信号放大系统，为科研人员提供稳定可靠的检测工具，适用于山羊繁殖生理机制研究、卵巢功能评估、生殖内分泌调控实验以及繁殖障碍模型的建立等科研场景，例如通过动态监测发情周期中LH浓度波动，探究排卵诱导机制或评估激素干预效果。该产品严格限定于科学研究用途，操作便捷且重复性良好，为畜牧繁殖研究及动物生殖生物学领域提供高效的技术支持。
缩写：

LH
种属：

Capra hircus (Goat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.3 mLU/mL-60 mLU/mL
灵敏度：

0.15 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-104
1:2	Average %	95
	Range %	90-103
1:4	Average %	91
	Range %	85-96
1:8	Average %	90
	Range %	88-93

回收率：

The recovery of goat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	92	87-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.