Rabbit luteinizing hormone (LH) ELISA kit

Rabbit luteinizing hormone (LH) ELISA kit

产品详情

引用文献

产品评价

靶点详情

产品详情

引用文献

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

兔子黄体生成素（LH）酶联免疫试剂盒
货号：

CSB-E17780Rb
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
相关产品
靶点详情

产品描述：

CUSABIO定量检测兔血清、血浆及组织匀浆样本中黄体生成素（LH）的夹心法酶联免疫试剂盒，货号CSB-E17780Rb，检测灵敏度为1.56 mIU/mL，线性范围覆盖1.56-100 mIU/mL，适用于科研场景中LH水平的精准分析。LH作为垂体前叶分泌的糖蛋白激素，通过调控性腺功能参与生殖周期、排卵及黄体形成等生理过程，是研究兔生殖生理、繁殖障碍模型及内分泌调控机制的重要靶标。试剂盒采用预包被特异性抗体的96孔板，通过双抗体夹心法捕获样本中的LH，结合酶标二抗与显色底物进行定量检测，操作流程包含样本预处理（血清/血浆需离心去沉淀，组织匀浆需稀释并离心取上清）、孵育反应及酶标仪读数。配套试剂包含标准品、检测抗体、显色液及终止液，建议样本避免反复冻融以保证检测稳定性。该产品可应用于兔繁殖生物学研究、激素水平动态监测、生殖疾病模型评估或药物干预实验中LH表达变化的分析，尤其适用于卵巢、垂体等组织局部LH表达水平的检测。
别名：

LHBLutropin subunit beta ELISA kit; Lutropin beta chain ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit
缩写：

LH
Uniprot No.：

Q6IY74
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 mIU/mL-100 mIU/mL
灵敏度：

0.39 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	90
	Range %	84-95
1:4	Average %	96
	Range %	89-102
1:8	Average %	98
	Range %	92-104

回收率：

The recovery of rabbit LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-104
EDTA plasma (n=4)	100	94-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
100	2.817	2.839	2.828	2.670
50	2.220	2.281	2.251	2.093
25	1.525	1.579	1.552	1.394
12.5	0.881	0.866	0.874	0.716
6.25	0.557	0.569	0.563	0.405
3.12	0.390	0.384	0.387	0.229
1.56	0.252	0.261	0.257	0.099
0	0.157	0.159	0.158

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Effects of Treatment with Nauclea latifolia Root Decoction on Sexual Behavior and Reproductive Functions in Male Rabbits Alabi QK.et al,J Diet Suppl,2017

功能：

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
基因功能参考文献：
1. This study determined the cDNA sequences of the luteinizing hormone beta-subunit (LHB) from Japanese White (JW), New Zealand White (NZW), and Dutch-Belted (Dutch) rabbits, and we compared these LHB sequences with those of other mammals. PMID: 17094992
亚细胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
数据库链接：

KEGG: ocu:100009427

STRING: 9986.ENSOCUP00000025405

UniGene: Ocu.3341

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.