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  4. Rat luteinizing hormone (LH) Elisa kit

Rat luteinizing hormone (LH) Elisa kit

文献引用 (125) 说明书下载
  • 中文名称：
    大鼠黄体生成素（LH）酶联免疫试剂盒
  • 货号：
    CSB-E12654r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠黄体生成素（LH）酶联免疫检测试剂盒（货号：CSB-E12654r），采用竞争法原理实现定量分析，适用于血清、血浆样本的科研检测。黄体生成素作为垂体前叶分泌的关键生殖激素，在排卵调控、性腺功能维持及类固醇合成中发挥重要作用，是研究大鼠生殖生理及内分泌调控机制的理想指标。本试剂盒检测范围为0.3-60 mlU/ml，最低检测灵敏度达0.3 mlU/ml，可精准捕捉样本中LH的浓度变化。实验采用预包被抗原的微孔板，通过样本中LH与标记抗体竞争结合的特性进行定量分析，操作流程包含样本预处理、孵育、显色及吸光度测定等标准化步骤。适用于科研领域探索生殖系统疾病模型建立、动情周期激素动态监测、药物干预对性腺轴的影响等研究场景。样本处理需注意血清/血浆需经离心分离，避免溶血或反复冻融影响检测准确性，建议实验前进行样本浓度预评估以确保结果可靠性。本试剂盒提供完整的标准品及检测溶液，实验结果需通过标准曲线计算获得，建议重复检测以提高数据稳定性。
  • 别名：
    LhbLutropin subunit beta ELISA Kit; Lutropin beta chain ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit
  • 缩写：
    LH
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.3 mlU/ml-60 mlU/ml
  • 灵敏度：
    0.15 mlU/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 88-100  
    1:2 Average % 95  
    Range % 91-101  
    1:4 Average % 94  
    Range % 85-105  
    1:8 Average % 96  
    Range % 90-102  
  • 回收率：
    The recovery of rat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-107  
    EDTA plasma (n=4) 96 91-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average    
    60 0.150 0.153 0.152    
    15 0.352 0.338 0.345    
    4.8 0.513 0.549 0.531    
    1.2 0.985 1.002 0.994    
    0.3 1.476 1.441 1.459    
    0 2.679 2.654 2.667    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Lhb Proteins

靶点详情

  • 最新研究进展：
    促黄体生成素是由垂体前叶分泌的蛋白质激素，对于女性生殖周期中的排卵和黄体生成起重要作用。最新的研究发现，LH还与男性生殖健康密切相关，可促进精子发生和成熟，并在精液中发挥重要作用。
  • 功能：
    Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
  • 基因功能参考文献：
    1. Orchestrated Egr1 and Pitx1 activities depending on specific central and peripheral regulatory inputs could be responsible for physiologically variable Lhb gene promoter activation in vivo. PMID: 25258388
    2. GnRH stimulates AMPK, and AMPK inhibition suppresses GnRH-stimulated LHbeta transcription. PMID: 23518923
    3. data suggest that the molecular decision for LHbeta subunit to enter the regulated secretory pathway is a pre-Golgi event controlled by the C-terminal heptapeptide. PMID: 23734233
    4. increased LH concentration induces production of incomplete LHR transcripts in hippocampal cells and decreases immature LHR at the protein level PMID: 23678542
    5. Data suggest that LH beta subunit expression (and FSH beta subunit expression) is regulated by DUSP1 expression (specifically in response to high-frequency GnRH pulses); this effect may occur in association with MAPK3/1 phosphorylation. PMID: 21228211
    6. Complex interactions between Ahr and Esr alter Prl and luteinizing hormone (LH) synthesis by direct actions in lactotropes and gonadotropes. PMID: 21187122
    7. LHbeta promoter activity is further regulated by the orphan nuclear receptors, chicken ovalbumin upstream promoter-transcription factors (COUP-TFI and COUP-TFII). PMID: 20797425
    8. data suggest that LHbeta G1502A polymorphism may be involved in the predisposition to infertility and minimal/mild endometriosis-associated infertility, although endometriosis might be only a coincidental finding along with infertility. PMID: 20430510
    9. PACAP may be released from the anterior pituitary cells in a concentration which would be able to influence LH release not only in vitro but under in vivo conditions as well. PMID: 12409218
    10. These results suggest that GnRH directly regulates LH secretion as well as LHbeta-subunit synthesis, and that LH2 cells are a useful model for the study of LH secretion induced by several secretagogues. PMID: 12940464
    11. androgens acting directly on the pituitary increase FSH-beta and decrease LH-beta transcription PMID: 14512439
    12. identify a second functional Pitx1 region spanning positions -73 to -52 in the LHbeta gene promoter PMID: 16087728
    13. JNK regulates both LHbeta and FSHbeta transcription in a differential manner. PMID: 17932215
    14. LHbeta, FSHbeta and the common alpha-subunit mRNA's were detected in intact follicles, theca cells, corpora lutea and in meiotically competent and incompetent oocytes. PMID: 18248883
    15. the carboxyl-terminal sequence in the lutropin beta subunit contributes to the sorting of lutropin to the regulated pathway PMID: 18292086
    16. In the 14-day-old rat pituitary, the majority of GHRH target cells are cells expressing LH beta mRNA alone or in combination with GH and/or POMC mRNA. PMID: 18363807
    17. Results demonstrate that degradation via the proteasome is vital to GnRH-stimulated LHbeta expression, and this occurs in part by allowing proper transcription factor associations with the LHbeta promoter. PMID: 19095772
    18. Lhb gene transcription in rat pituitary requires GnRH-induced early growth response 1 gene expression, which is mediated by MAPK8/9 and MAPK1/3. PMID: 19710510

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 数据库链接：

    KEGG: rno:25329

    STRING: 10116.ENSRNOP00000060360

    UniGene: Rn.10108

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |