Bovine luteinizing hormone (LH) ELISA kit

Bovine luteinizing hormone (LH) ELISA kit

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中文名称：

牛黄体生成素（LH）酶联免疫试剂盒
货号：

CSB-E12826B
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO牛黄体生成素（LH）酶联免疫检测试剂盒（货号：CSB-E12826B），基于竞争法原理实现定量分析，适用于血清、血浆及组织匀浆样本的LH水平检测。LH作为垂体前叶分泌的糖蛋白激素，在动物生殖周期调控中起核心作用，参与排卵诱导和黄体形成等关键生理过程，是畜牧业繁殖管理及生殖内分泌研究的重要指标。CUSABIO试剂盒采用高灵敏度检测体系，可精准检测1.25至100 mIU/mL浓度范围的LH，配套标准品和预包被板可实现样本中目标蛋白的快速定量分析，其竞争法设计能有效降低样本基质干扰，特别适合含有复杂成分的生物样本检测。该试剂盒广泛应用于动物繁殖生理机制研究、发情周期监测、生殖激素调控实验等科研领域，亦可用于评估饲料添加剂或环境因素对牛群生殖功能的影响，为畜牧养殖优化和基础医学研究提供可靠数据支持。
别名：

LHBLutropin subunit beta ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit; Lutropin beta chain ELISA kit
缩写：

LH
Uniprot No.：

P04651
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.25 mIU/mL-100 mIU/mL
灵敏度：

1.56 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	95-102
1:2	Average %	87
	Range %	84-90
1:4	Average %	88
	Range %	83-94
1:8	Average %	100
	Range %	95-105

回收率：

The recovery of bovine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-95
EDTA plasma (n=4)	87	85-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average
100	0.361	0.368	0.365
37.5	0.538	0.567	0.553
18.75	0.781	0.765	0.773
6.25	1.156	1.126	1.141
1.25	1.662	1.698	1.680
0	2.161	2.178	2.170

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Exploring the impact of exogenous Kisspeptin-10 on ovarian follicular development and estrus behaviour in Lakhimi cows of Assam A DATTA,/,2024
Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum Y Wang,researchsquare,2022
Epitope-based in silico peptide design yields peptide-directed antibodies that recognize the buffalo luteinizing hormone V Nayan,International Journal of Biological Macromolecules,2021
A study on stress response and fertility parameters in phenotypically thermotolerant and thermosensitive dairy cows during summer heat stress I Nanas,Reprod Domest Anim,2020
Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones Umer S, et al,Genes,2019
Association among lipopolysaccharide, the transforming growth factor-beta superfamily, follicular growth, and transcription factors in spontaneous bovine ovarian cysts Colakoglu H E, et al,Domestic Animal Endocrinology,2019
Urine levels of luteinizing hormone as predictor of the period of ovulation for advantage of timed-artificial insemination in murrah buffalo (Bubalus bubalis) RM Selvam..et al,REPRODUCTION IN DOMESTIC ANIMALS,2017
Exploration of Luteinizing hormone in murrah buffalo (Bubalus bubalis) urine: Extended surge window opens door for estrus prediction Ramu Muthu Selvam.et al,General and Comparative Endocrinology,2016

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功能：

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
亚细胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
数据库链接：

KEGG: bta:280839

STRING: 9913.ENSBTAP00000048635

UniGene: Bt.12926

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.