Chicken Epidermal growth factor,EGF ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO鸡表皮生长因子(EGF)酶联免疫检测试剂盒（货号CSB-E09868Ch），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中EGF含量，适用于家禽生长调控、组织修复等基础研究领域。EGF作为一种重要的细胞增殖调节因子，在胚胎发育、上皮细胞再生及创伤愈合过程中发挥关键作用，该试剂盒可精准检测125-8000 pg/mL浓度范围内的目标蛋白，最低检测限为50 pg/mL。试剂盒包含预包被特异性抗体的96孔板、标准品、检测抗体及显色底物系统，操作全程约需4.5小时，其中包含1小时37℃孵育步骤。科研人员可通过该试剂盒动态监测实验动物EGF分泌水平，用于家禽生理机制研究、营养干预实验或疾病模型中的生长因子表达分析。本产品需配合酶标仪在450nm波长下读数，所有组分均经过严格验证，确保批次间稳定性，建议实验时设置复孔以提高数据可靠性。
别名：

N/A
缩写：

EGF
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, tissue homogenates
检测范围：

125 pg/mL-8000 pg/mL
灵敏度：

31.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	100-104
1:2	Average %	95
	Range %	92-98
1:4	Average %	88
	Range %	85-91
1:8	Average %	99
	Range %	96-102

回收率：

The recovery of chicken EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	102-106
EDTA plasma (n=4)	93	90-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.