Pig Epithelial growth factor,EGF ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪表皮生长因子（EGF）酶联免疫检测试剂盒（货号CSB-E06788p），采用双抗体夹心法定量分析血清、血浆或组织匀浆样本中的EGF浓度，检测范围为250-10000 pg/mL。EGF是一种多功能的细胞调控因子，通过结合表皮生长因子受体参与细胞增殖、分化及组织修复等过程，对研究猪的生长发育、代谢调节及炎症反应等具有重要意义。试剂盒基于特异性抗体对靶蛋白的捕获与检测，提供高灵敏度与稳定性，适用于实验室环境下检测猪源性生物样本中EGF的表达水平，可广泛应用于动物营养学、生殖生理学、免疫学等基础研究领域，例如探究饲料添加剂对肠道黏膜修复的影响、评估应激状态下EGF的动态变化，或解析特定疾病模型中生长因子调控机制。操作流程标准化，兼容常规酶标仪分析，为科研工作者提供可靠工具。
缩写：

EGF
Uniprot No.：

F1S137
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

250 pg/mL-10000 pg/mL
灵敏度：

125 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-105
1:2	Average %	93
	Range %	90-96
1:4	Average %	102
	Range %	98-106
1:8	Average %	87
	Range %	83-90

回收率：

The recovery of pig EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	101	99-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.