Bovine Epithelial growth factor(EGF)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO牛表皮生长因子（EGF）酶联免疫检测试剂盒（货号：CSB-E17171B），基于双抗体夹心法原理实现精准定量分析，适用于牛血清及血浆样本中EGF蛋白的检测。表皮生长因子是一种多功能细胞调控因子，在细胞增殖、分化及组织修复等生理过程中发挥重要作用，其水平变化与动物生长代谢及健康状态密切相关。试剂盒检测灵敏度达0.156 ng/mL，线性检测范围为0.156-10 ng/mL，通过优化包被抗体与检测抗体的特异性组合，确保对目标蛋白的高效捕获与信号放大。该产品可广泛应用于动物生理学研究、营养代谢评估、疾病模型构建等科研领域，为探究牛EGF在生长发育调控、免疫应答及病理过程中的分子机制提供可靠工具。试剂盒包含预包被96孔板、标准品、检测抗体及显色系统，实验流程经过标准化验证，操作简便且重复性良好，满足实验室高通量检测需求。
别名：

HOMG4, URG, beta-urogastrone|epidermal growth factor
缩写：

EGF
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-97
1:2	Average %	106
	Range %	100-109
1:4	Average %	90
	Range %	85-94
1:8	Average %	94
	Range %	90-97

回收率：

The recovery of bovine EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	94	89-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.