Rabbit Epidermal growth factor,EGF ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO科研用兔表皮生长因子(EGF)酶联免疫试剂盒（货号CSB-E06909Rb），通过竞争法检测模式实现对生物样本中EGF蛋白的精确定量分析。EGF作为一种重要的细胞增殖调控因子，广泛参与皮肤修复、黏膜再生等生理过程，其浓度变化与组织再生能力及病理状态密切相关。该试剂盒检测灵敏度达0.5 ng/mL，线性范围覆盖0.5-8 ng/mL，可稳定检测血清、血浆及组织匀浆等样本类型，特别适用于探究EGF在皮肤屏障功能、消化道黏膜修复、创伤愈合等研究领域的调控机制。实验操作采用96孔板体系，配备标准品和特异性抗体，通过优化的反应体系确保数据可靠性。科研人员可利用该试剂盒分析不同生理/病理条件下体液和组织中的EGF动态变化，为研究生长因子在再生医学、肿瘤微环境中的作用提供量化依据，其宽检测范围设计可有效应对复杂生物样本中目标蛋白浓度的显著波动。
缩写：

EGF
Uniprot No.：

G1TBJ6
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.5 ng/mL-8 ng/mL
灵敏度：

0.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	111
	Range %	100-115
1:2	Average %	92
	Range %	84-97
1:4	Average %	100
	Range %	90-104
1:8	Average %	100
	Range %	93-104

回收率：

The recovery of rabbit EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	92	87-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.