BTLA

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized BTLA at 5 μg/ml can bind biotinylated human TNFRSF14 (CSB-MP842173HU-A), the EC₅₀ is 137.8-233.4 ng/ml.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human BTLA at 2μg/ml can bind Anti-BTLA recombinant antibody(CSB-RA773799MAIHU), the EC₅₀ is 1.763-3.542 ng/mL.

The Binding Activity of Human BTLA with Anti-BTLA Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human BTLA(CSB-MP773799HU1)at 2 μg/mL can bind Anti-BTLA recombinant antibody , the EC₅₀ is 11.56-13.00 ng/mL.

Untransfected Jurkat cells surface (green line) and transfected Human BTLA Jurkat stable cells surface (red line) were stained with anti-BTLA antibody (2µg/1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Untransfected CHO-K1*10⁶cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.