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Human platelet activating factor,PAF ELISA Kit

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CUSABIO人血小板活化因子（PAF）酶联免疫检测试剂盒（货号：CSB-E07929h），采用双抗体夹心原理实现定量分析，适用于检测血清、尿液、细胞培养上清液、组织匀浆及细胞裂解液等多种样本类型。PAF是一种具有生物活性的磷脂介质，在炎症反应、过敏应答、血小板活化及血栓形成等病理生理过程中发挥重要作用，其水平变化与心血管疾病、免疫紊乱等研究密切相关。本试剂盒检测线性范围为4.69-300 ng/mL，最低检测限为1.17 ng/mL，实验全程约需4.5小时，包含预包被捕获抗体、生物素标记检测抗体及标准品等核心组分。该产品可为心血管疾病机制研究、炎症反应模型构建、药物靶点筛选等科研领域提供PAF浓度的精准检测，尤其适用于体外实验模型中PAF动态变化的定量分析，帮助研究者深入探究其在细胞信号传导、免疫调控及病理过程中的作用机制。
别名：

N/A
缩写：

PAF
种属：

Homo sapiens (Human)
样本类型：

serum, urine, cell culture supernates, tissue homogenates, cell lysates
检测范围：

4.69 ng/mL-300 ng/mL
灵敏度：

1.17 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-92
1:2	Average %	95
	Range %	90-105
1:4	Average %	94
	Range %	91-102
1:8	Average %	90
	Range %	80-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.097	2.093	2.095	1.994
150	1.823	1.793	1.808	1.707
75	1.476	1.374	1.425	1.324
37.5	1.029	0.986	1.008	0.907
18.75	0.782	0.772	0.777	0.676
9.38	0.421	0.401	0.411	0.310
4.69	0.218	0.202	0.210	0.109
0	0.105	0.097	0.101

本试剂盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human PAF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled PAF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-conjugateDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.