Rat platelet activating factor,PAF ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠血小板活化因子（PAF）酶联免疫检测试剂盒（货号CSB-E07930r），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的PAF水平，检测灵敏度为1.56 ng/mL，线性范围为1.56-100 ng/mL。PAF是一种重要的磷脂类炎症介质，参与血小板活化、血管通透性调节及炎症反应等生理病理过程，与血栓形成、哮喘、败血症等疾病机制密切相关。本试剂盒通过特异性捕获抗体和检测抗体构建高精度检测体系，适用于科研场景下对大鼠模型中PAF表达的动态分析，例如评估炎症性疾病动物模型的PAF释放水平、研究药物或基因干预对PAF信号通路的影响，或探索PAF在心血管、免疫相关疾病中的作用机制。试剂盒操作流程标准化，可在3-4小时内完成检测，配套提供预包被96孔板、标准品、显色试剂及终止液等完整组分，确保实验便捷性和结果重复性，为体外实验和动物研究提供可靠的PAF定量工具。
别名：

N/A
缩写：

PAF
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	92
	Range %	87-98
1:4	Average %	89
	Range %	86-98
1:8	Average %	104
	Range %	96-108

回收率：

The recovery of rat PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-92
EDTA plasma (n=4)	92	88-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.