Mouse platelet activating factor,PAF ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于科研领域的小鼠血小板活化因子（PAF）酶联免疫检测试剂盒，采用双抗体夹心法原理实现定量检测，适用于血清、血浆、细胞培养上清及组织匀浆样本的分析。PAF作为一种重要的脂质介质，参与炎症反应、过敏反应及血小板活化等生理病理过程，其水平变化与多种疾病机制研究密切相关。本试剂盒检测灵敏度高，可精准检测0.78 ng/mL至50 ng/mL浓度范围的PAF，最低检测限为0.19 ng/mL，标准品采用梯度冻干粉形式，包含0、0.78、1.56、3.12、6.25、12.5、25、50 ng/mL共8个浓度点。试剂盒提供预包被捕获抗体的96孔板、生物素标记检测抗体及标准品等核心组分，操作流程涵盖样本孵育、抗体结合、酶标反应及显色测定步骤，实验全程约需4.5小时完成。该试剂盒广泛应用于心血管疾病、免疫调节、炎症模型构建等基础研究领域，尤其适用于体外实验中PAF动态变化的定量监测，为探索相关信号通路及药物作用靶点提供可靠工具。
别名：

N/A
缩写：

PAF
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	97
	Range %	92-107
1:4	Average %	93
	Range %	87-98
1:8	Average %	87
	Range %	82-94

回收率：

The recovery of mouse PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	97	95-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.