Human Interleukin 23,IL-23 ELISA Kit

Human Interleukin 23,IL-23 ELISA Kit

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中文名称：

人白介素23(IL-23)酶联免疫试剂盒
货号：

CSB-E08461h
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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人白介素23(IL-23)酶联免疫试剂盒（CSB-E08461h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-23含量。IL-23是重要治疗靶点。它是一种促炎细胞因子，在自身免疫病发病机制中起关键作用。研究发现阻断IL-23信号通路可调节免疫反应，有望为银屑病、炎症性肠病等疾病治疗带来新方法，目前相关药物研发是热点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，科研人员可将其用于免疫机制研究、炎症性疾病模型分析、药物靶点筛选等基础研究领域，特别适用于评估实验动物或细胞模型中IL - 23的表达变化及其在病理生理过程中的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

IL-23
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	98
	Range %	92-106
1:4	Average %	94
	Range %	90-98
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of human IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-96
EDTA plasma (n=4)	93	89-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.608	2.507	2.558	2.415
200	1.865	1.764	1.815	1.672
100	1.207	1.186	1.197	1.054
50	0.801	0.790	0.796	0.653
25	0.437	0.448	0.443	0.300
12.5	0.301	0.312	0.307	0.164
6.25	0.228	0.234	0.231	0.088
0	0.142	0.143	0.143

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Profiling of IgG N-glycosylation for axial spondyloarthritis and other rheumatic diseases X Xu, Z Chen, M Song, Z Hou,Arthritis Research & Therapy,2025
METTL14-m6A-FOXO3a axis regulates autophagy and inflammation in ankylosing spondylitis Y Chen,Clinical immunology,2023
Study of serum some immune facter of CD4 and IL-23 indicatore on thalassemia patients in Abn Albaladiu hospital in Baghdad IH Mohammed,AIP Publishing LLC,2023
Interleukin-17, Interleukin-23 and Alkalinephosphatase Levels in Patients with Helicobacter pylori Infection KIA Mohammed,South Asian Res J Bio Appl Biosci,2022
Protective Roles of tRNA‐Derived Small RNA tRF‐Ile‐AAT‐019 in Pathological Progression of Psoriasis Z Jinrong,Experimental Dermatology,2022
Serum Levels of Il-12 and Il-23 in Breast Cancer Patients Infected with Toxoplasma gondii: A Case-Control Study Wafaa Mohammed ElZefzafy,iranian journal parasitology,2020
H19 increases IL-17A/IL-23 releases via regulating VDR by interacting with miR675-5p/miR22-5p in ankylosing spondylitis Zhang X, et al,Molecular Therapy-Nucleic Acids,2019
Comparison of pleural effusion features and biomarkers between talaromycosis and tuberculosis in non-human immunodeficiency virus-infected patients Qiu Y, et al,Bmc Infectious Diseases,2019
Genistein suppresses psoriasis-related inflammation through a STAT3-NF-κB-dependent mechanism in keratinocytes Wang A, et al,International Immunopharmacology,2019
Evaluation of IL-23 concentration in a miscarriages woman Infected with Toxoplasma gondii Raad Ajam Sayal, et al,/,2018
The Association of Severe Toxoplasmosis and Some Cytokine Levels in Breast Cancer Patients Maha Mustafa Assim.et al,Iraqi Journal of Science,2018
Up-regulated lncRNA-MSX2P1 promotes the growth of IL-22-stimulated keratinocytes by inhibiting miR-6731-5p and activating S100A7 Meng Qiao.et al,Experimental Cell Research,2018
Peripheral blood mononuclear cells of patients with latent autoimmune diabetes secrete higher levels of pro- & anti-inflammatory cytokines compared to those with type-1 diabetes mellitus following in vitro stimulation with β-cell autoantigens. Badal D.et al,Indian J Med Res. ,2017
Women with recurrent spontaneous abortion have decreased 25(OH) vitamin D and VDR at the fetal-maternal interface Li N.et al,Braz J Med Biol Res,2017
Dexmedetomidine Inhibits Maturation and Function of Human Cord Blood-Derived Dendritic Cells by Interfering with Synthesis and Secretion of IL-12 and IL-23 Chen G.et al,PLoS One.,2016
Distinct intrathecal interleukin-17/interleukin-6 activation in anti-N-methyl-d-aspartate receptor encephalitis Byun J I.et al,Journal of Neuroimmunology,,2016

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■ 常见问题解答

I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??

Thanks for your inquiry. For these cytokines IL-23, IL-21, IL-22, IL-17A, IL-17F,detection of cross-species is not advised,as it will be affected by the characteristics of antibodies and the matrix difference.
Regarding your research in measuring cytokines in patients affected by autoimmune thyroid diseases,we suggest you use human kits.While regarding your early laboratory research on lab animals,we suggest you use the corresponding lab animals kits.
Human kits:
#CSB-E08461h,Human Interleukin 23,IL-23 ELISA Kit
#CSB-E11707h,Human Interleukin 21,IL-21 ELISA Kit
#CSB-E13418h,Human Interleukin 22(IL-22) ELISA KIT
#CSB-E12819hHuman Interleukin 17A (IL-17A/IL-17) ELISA Kit
#CSB-E15916h,Human Interleukin17F (IL17F) ELISA Kit
Mouse kits:
#CSB-E08463m,Mouse Interleukin 23,IL-23 ELISA Kit
#CSB-E13162m,Mouse Interleukin 21(IL-21) ELISA Kit
#CSB-E13513m,Mouse Interleukin 22(IL-22) ELISA Kit
#CSB-E04608m,Mouse Interleukin 17,IL-17 ELISA Kit
#CSB-EL011601MO,Mouse Interleukin-17F(IL17F) ELISA kit
Rat kits:
#CSB-E08462r,Rat Interleukin 23,IL-23 ELISA Kit
#CSB-E11843r,Rat Interleukin 21,IL-21 ELISA Kit
#CSB-E14918r,Rat Interleukin 22(IL-22) ELISA Kit
#CSB-E07451r,Rat Interleukin 17,IL-17 ELISA KIT
#CSB-EL011601RA,Rat Interleukin-17F(IL17F) ELISA kit
If you are interested to order any one of them,we will foward your request to local distributor.Well,could you advise which coutry are you from?Thank you very much!

I am interested in this kit. I would like to know which subunit of IL-23 can be detected by the kit.
Please provide a detailed information about kit’s components:
a. Capture antibody or capture antigen.
b. Detection antibody (if applicable).
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.

Thanks for your inquiry.
Interleukin-23 (IL-23) is a heterodimeric cytokine composed of an IL-12p40 subunit (that is shared with IL-12 ) and the IL-23p19 subunit.
CSB-E08461h
This kit detects IL23（it is compound）.
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is a dimer of recombinant proteins expressed by eukaryotic cells in vitro.

Thank you for your assistance.
Is the 293 protein you are referring the IL-23 p19 subunit?
Because you just mention the immunogen is 293 cell-derived recombinant protein. Please clarify, what is the 293 cell-derived protein specifically? For example, is it the IL-23 p19 subunit?
It is good to know, if the antibodies bind to the p40 unit or the p19 subunit?
Looking forward to receiving your feedback.

Thanks for your further inquiry. There are 2 antibodies in the kit, so there are 2 immunogens. They are designed against the 2 subunits of the compound.
Sorry we can not disclose which antibody is specific to which protein.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.