Rat Interleukin 23,IL-23 ELISA Kit

Rat Interleukin 23,IL-23 ELISA Kit

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中文名称：

大鼠白介素23(IL-23)酶联免疫试剂盒
货号：

CSB-E08462r
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO大鼠白介素23（IL-23）酶联免疫检测试剂盒（货号CSB-E08462r），采用双抗体夹心法定量分析血清、血浆、细胞培养上清及组织匀浆样本中的IL-23含量。IL-23是白介素家族中参与炎症与免疫调节的关键细胞因子，通过促进Th17细胞分化在自身免疫性疾病及感染性疾病的病理机制中发挥重要作用。本试剂盒检测灵敏度高，线性检测范围为3.12-200 pg/mL，可精准识别低浓度目标蛋白，适用于体外炎症模型研究、免疫通路调控实验或药物干预效果评估等科研领域。实验操作流程标准化，配备预包被捕获抗体、生物素标记检测抗体及HRP-链霉亲和素信号放大系统，支持96孔板批量检测，兼容多种生物样本类型，为探索IL-23在神经免疫、肠道屏障功能或慢性炎症反应中的分子机制提供可靠工具。
别名：

N/A
缩写：

IL-23
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-94
1:2	Average %	104
	Range %	100-107
1:4	Average %	92
	Range %	87-95

回收率：

The recovery of rat IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-95
EDTA plasma (n=4)	98	94-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.147	2.112	2.130	1.998
100	1.851	1.776	1.814	1.682
50	1.308	1.288	1.298	1.166
25	0.903	0.898	0.901	0.769
12.5	0.621	0.614	0.618	0.486
6.25	0.375	0.359	0.367	0.235
3.12	0.236	0.224	0.230	0.098
0	0.134	0.130	0.132

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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Mesenchymal stem cells moderate experimental autoimmune uveitis by dynamic regulating Th17 and Breg cells response Yajun Mu,Journal of Tissue Engineering and Regenerative Medicine,2021
The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells J Ding,Evidence-Based Complementary and Alternative Medicine,2020
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Synthetic p55 tandem DNA vaccine against Pneumocystis carinii in rats. Fan H.et al,Microbiol Immunol.,2016
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Mast cells mediate Pseudomonas aeruginosa lipopolysaccharide-induced lung inflammation in rat B. V. Lê,Tumor Biology,2012
Modulation of Pseudomonas aeruginosa lipopolysaccharide-induced lung inflammation by chronic iron overload in rat Bá Vuong Lê,International Journal of Rheumatic Diseases,2012

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■ 常见问题解答

I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??

Thanks for your inquiry. For these cytokines IL-23, IL-21, IL-22, IL-17A, IL-17F,detection of cross-species is not advised,as it will be affected by the characteristics of antibodies and the matrix difference.
Regarding your research in measuring cytokines in patients affected by autoimmune thyroid diseases,we suggest you use human kits.While regarding your early laboratory research on lab animals,we suggest you use the corresponding lab animals kits.
Human kits:
#CSB-E08461h,Human Interleukin 23,IL-23 ELISA Kit
#CSB-E11707h,Human Interleukin 21,IL-21 ELISA Kit
#CSB-E13418h,Human Interleukin 22(IL-22) ELISA KIT
#CSB-E12819hHuman Interleukin 17A (IL-17A/IL-17) ELISA Kit
#CSB-E15916h,Human Interleukin17F (IL17F) ELISA Kit
Mouse kits:
#CSB-E08463m,Mouse Interleukin 23,IL-23 ELISA Kit
#CSB-E13162m,Mouse Interleukin 21(IL-21) ELISA Kit
#CSB-E13513m,Mouse Interleukin 22(IL-22) ELISA Kit
#CSB-E04608m,Mouse Interleukin 17,IL-17 ELISA Kit
#CSB-EL011601MO,Mouse Interleukin-17F(IL17F) ELISA kit
Rat kits:
#CSB-E08462r,Rat Interleukin 23,IL-23 ELISA Kit
#CSB-E11843r,Rat Interleukin 21,IL-21 ELISA Kit
#CSB-E14918r,Rat Interleukin 22(IL-22) ELISA Kit
#CSB-E07451r,Rat Interleukin 17,IL-17 ELISA KIT
#CSB-EL011601RA,Rat Interleukin-17F(IL17F) ELISA kit

If you are interested to order any one of them,we will foward your request to local distributor.Well,could you advise which coutry are you from?Thank you very much!

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.