Mouse Interleukin 23,IL-23 ELISA Kit

中文名称：

小鼠白介素23(IL-23)酶联免疫试剂盒
货号：

CSB-E08463m
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠白介素23（IL-23）定量检测专用ELISA试剂盒（货号CSB-E08463m），采用双抗体夹心法原理，可精准检测血清、血浆及组织匀浆样本中的IL-23蛋白浓度。IL-23作为促炎性细胞因子，在Th17细胞分化及自身免疫性疾病研究中具有重要作用。试剂盒检测范围覆盖7.8-500 pg/ml，最低检测限达7.8 pg/ml，配套预包被抗体的96孔板、标准品、生物素标记检测抗体及显色底物，通过链霉亲和素-HRP系统实现高灵敏度检测。适用于科研领域中对感染性疾病模型、炎症反应机制、免疫调节研究的IL-23动态监测，也可用于评估药物或生物制剂对IL-23通路的影响。实验操作流程包含样本预处理、标准品梯度稀释、抗体孵育及酶标仪检测步骤，提供标准曲线建立与浓度计算方案，满足实验室对IL-23蛋白表达水平的定量分析需求。
别名：

N/A
缩写：

IL-23
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 pg/ml-500 pg/ml
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-92
1:2	Average %	95
	Range %	89-100
1:4	Average %	93
	Range %	84-97
1:8	Average %	106
	Range %	97-110

回收率：

The recovery of mouse IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	94-108
EDTA plasma (n=4)	101	94-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.868	2.689	2.779	2.654
250	1.519	1.586	1.553	1.428
125	0.746	0.700	0.723	0.598
62.5	0.434	0.456	0.445	0.320
31.2	0.314	0.301	0.308	0.183
15.6	0.207	0.202	0.205	0.080
7.8	0.153	0.159	0.156	0.031
0	0.127	0.123	0.125

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

QingChang-XiaoPi Decoction Ameliorates Intestinal Inflammation of Ulcerative Colitis by Regulating the Pathogenicity of Th17 Cells R Jia,Phytomedicine,2024
Novel bispecific nanobody mitigates experimental intestinal inflammation in mice by targeting TNF‐α and IL‐23p19 bioactivities J Wang,Clinical and translational medicine,2024
Tanshinone IIA, a component of the self‐made Xiao‐Yin decoction, ameliorates psoriasis by inhibiting IL‐17/IL‐23 and PTGS2/NF‐κB/AP‐1 pathways G Zou,Skin research and technology,2024
IFN-r induced by IL-12 administration prevents diabetes by inhibiting pathogenic IL-17 production in NOD mice Jun Zhang et al,Journal of Autoimmunity,2012

产品评价

平均分:

5.0分 - 1 个评价

样品类型：组织匀浆液海马组织

样品信息：小鼠

稀释比：1:50

产品评价：我用CSB-E08463m分别检测了磁刺激前后小鼠海马组织匀浆中IL-23水平，标曲结果良好，发现BTBR小鼠IL-23水平高于C57小鼠，磁刺激后IL-23水平降低，与预期结果一致。试剂盒操作方便。

By 王老师

相关问答

■ 常见问题解答

I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??

Thanks for your inquiry. For these cytokines IL-23, IL-21, IL-22, IL-17A, IL-17F,detection of cross-species is not advised,as it will be affected by the characteristics of antibodies and the matrix difference.
Regarding your research in measuring cytokines in patients affected by autoimmune thyroid diseases,we suggest you use human kits.While regarding your early laboratory research on lab animals,we suggest you use the corresponding lab animals kits.
Human kits:
#CSB-E08461h,Human Interleukin 23,IL-23 ELISA Kit
#CSB-E11707h,Human Interleukin 21,IL-21 ELISA Kit
#CSB-E13418h,Human Interleukin 22(IL-22) ELISA KIT
#CSB-E12819hHuman Interleukin 17A (IL-17A/IL-17) ELISA Kit
#CSB-E15916h,Human Interleukin17F (IL17F) ELISA Kit
Mouse kits:
#CSB-E08463m,Mouse Interleukin 23,IL-23 ELISA Kit
#CSB-E13162m,Mouse Interleukin 21(IL-21) ELISA Kit
#CSB-E13513m,Mouse Interleukin 22(IL-22) ELISA Kit
#CSB-E04608m,Mouse Interleukin 17,IL-17 ELISA Kit
#CSB-EL011601MO,Mouse Interleukin-17F(IL17F) ELISA kit
Rat kits:
#CSB-E08462r,Rat Interleukin 23,IL-23 ELISA Kit
#CSB-E11843r,Rat Interleukin 21,IL-21 ELISA Kit
#CSB-E14918r,Rat Interleukin 22(IL-22) ELISA Kit
#CSB-E07451r,Rat Interleukin 17,IL-17 ELISA KIT
#CSB-EL011601RA,Rat Interleukin-17F(IL17F) ELISA kit

If you are interested to order any one of them,we will foward your request to local distributor.Well,could you advise which coutry are you from?Thank you very much!

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Mouse Interleukin 23,IL-23 ELISA Kit

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