Mouse parathyroid hormone (PTH) ELISA kit

产品详情

产品描述：

CUSABIO小鼠甲状旁腺激素(PTH)酶联免疫检测试剂盒（货号：CSB-E08690m），采用双抗体夹心法原理实现定量检测，适用于血清和组织匀浆样本中的PTH蛋白分析。甲状旁腺激素是调节钙磷代谢的关键激素，通过作用于骨骼、肾脏等靶器官维持血钙平衡，其异常表达与多种钙代谢紊乱疾病相关。本试剂盒检测灵敏度为6.25 pg/mL，线性检测范围为6.25-400 pg/mL，可精准检测小鼠模型中PTH的生理浓度波动。试剂盒包含预包被捕获抗体的96孔板、生物素标记检测抗体及标准品等全套组分，实验流程包含样本孵育、信号放大和比色测定三个核心步骤，配合酶标仪在450nm波长下完成数据读取。该产品适用于基础研究中的钙代谢调控机制探索、甲状旁腺功能相关疾病模型建立、药物干预效果评估等方向，特别针对骨代谢研究、肾性骨营养不良模型分析及内分泌系统相关实验提供可靠检测工具，为科研人员解析PTH在生理病理过程中的作用机制提供标准化解决方案。
缩写：

PTH
种属：

Mus musculus (Mouse)
样本类型：

serum, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-99
1:2	Average %	100
	Range %	96-104
1:4	Average %	90
	Range %	84-94
1:8	Average %	96
	Range %	89-100

回收率：

The recovery of mouse PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-95
EDTA plasma (n=4)	104	97-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.