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  4. Bovine Parathyroid hormone(PTH) ELISA kit

Bovine Parathyroid hormone(PTH) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    牛甲状旁腺素(PTH)酶联免疫试剂盒
  • 货号：
    CSB-EL018987BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    牛甲状旁腺素(PTH)酶联免疫试剂盒（CSB-EL018987BO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PTH含量。甲状旁腺激素（PTH）是甲状旁腺主细胞分泌的肽类激素。它对钙磷代谢具有关键调节作用。研究机制方面，主要通过作用于骨和肾脏，促进骨钙释放、增加肾小管对钙的重吸收，维持血钙水平稳定，且在骨代谢相关疾病研究中受关注。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于科研场景中牛PTH相关机制研究，如钙代谢调控、骨骼发育分析、营养代谢评估及内分泌疾病模型构建等领域，为畜牧养殖、动物生理学及基础医学研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
  • 缩写：
    PTH
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 86-99
    1:2 Average % 96
    Range % 90-110
    1:4 Average % 90
    Range % 83-99
    1:8 Average % 95
    Range % 85-105
  • 回收率：
    The recovery of bovine PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 88-98
    EDTA plasma (n=4) 96 90-110
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.626 2.603 2.615 2.498
    1000 2.365 2.325 2.345 2.228
    500 1.986 1.992 1.989 1.872
    250 1.502 1.486 1.494 1.377
    125 0.841 0.835 0.838 0.721
    62.5 0.486 0.474 0.480 0.363
    31.25 0.371 0.375 0.373 0.256
    0 0.115 0.119 0.117 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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PTH Proteins

靶点详情

  • 功能：
    PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • 基因功能参考文献：
    1. These data highlight the ability of PTH to phosphorylate beta-catenin directly via PKA. PMID: 26851123
    2. These data suggest that prostaglandin E2 acting via EP4R on bone marrow macrophages committed to the osteoblast cell lineage, stimulated secretion of a factor or factors that acted to suppress PTH-stimulated osteoblast differentiation. PMID: 23639875
    3. These results suggest that in vivo PTH treatment increased in vitro osteoclastogenesis and resorption without altering the number of osteoclast precursors. PMID: 21611963
    4. Osteoblast 2-deoxyglucose uptake and glycogen synthesis were increased after exposure to low concentrations (0.1 nmol/l and above) of PTH. PMID: 21076856
    5. Parathyroid hormone stimulated growth and decreased Col-X deposition via phosphotidylinositol-3,4,5 triphosphate kinase and mitogen activating protein kinase pathways in avian sterna. PMID: 19957341
    6. likely involvement of the Sp family in regulating PTH gene expression through interactions with an Sp1 DNA element in the hormone's promoter. PMID: 12810570
    7. alternative cis-acting protein-binding elements may determine the regulation of PTH mRNA stability in response to changes in serum calcium and phosphate PMID: 15824859
    8. a possible role for the Wnt signaling pathway in PTH actions in bone PMID: 15962290
    9. Data suggest that calcium-mediated destabilization of parathyroid hormone mRNA requires gene transcription and involves increases in cytosolic Ca. PMID: 18096993

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Parathyroid hormone family
  • 数据库链接：

    KEGG: bta:280903

    STRING: 9913.ENSBTAP00000025399

    UniGene: Bt.95

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |