Chicken Parathyroid hormone,PTH ELISA Kit

Chicken Parathyroid hormone,PTH ELISA Kit

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中文名称：

鸡甲状旁腺激素(PTH)酶联免疫试剂盒
货号：

CSB-E11880Ch
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO鸡甲状旁腺激素（PTH）酶联免疫吸附试验（ELISA）科研试剂盒（货号：CSB-E11880Ch），基于竞争法原理实现定量检测，适用于分析血清、血浆及组织匀浆样本中PTH的表达水平。甲状旁腺激素作为禽类钙磷代谢的核心调节因子，在维持血钙稳态、骨骼代谢及肾脏功能中发挥关键作用，其检测可为禽类生理调控、营养代谢及内分泌相关研究提供重要依据。试剂盒检测灵敏度高，线性范围为6.25-100 pg/mL，能够精确捕捉样本中微量PTH的动态变化。实验操作采用预包被抗体的96孔板体系，通过生物素标记抗体与样本中目标蛋白的竞争性结合反应，结合显色系统实现信号检测。该产品广泛应用于禽类钙代谢机制研究、饲料营养评估、疾病模型构建等科研场景，尤其适用于探究环境因素、遗传变异或药物干预对禽类甲状旁腺功能的影响。所有检测结果需配合标准曲线进行定量分析，实验过程需严格按照说明书操作以确保数据可靠性。本试剂盒仅限科学研究使用。
别名：

PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit
缩写：

PTH
Uniprot No.：

P15743
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-100 pg/mL
灵敏度：

6.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-95
1:2	Average %	96
	Range %	92-103
1:4	Average %	85
	Range %	81-86
1:8	Average %	90
	Range %	83-98

回收率：

The recovery of chicken PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
100	0.184	0.195	0.190
50	0.325	0.350	0.338
25	0.531	0.504	0.518
12.5	1.006	1.026	1.016
6.25	2.117	2.220	2.169

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Effects of a transient lack of dietary mineral phosphorus on renal gene expression and plasma metabolites in two high-yielding laying hen strains H Qasir, H Reyer, M Oster, S Ponsuksili, N Trakooljul,BMC genomics,2025
Dietary Clostridium butyricum and 25-Hydroxyvitamin D3 modulate bone metabolism of broilers through the gut–brain axis G Cao,Poultry science,2024
Circadian regulation of calcium and phosphorus homeostasis during the oviposition cycle in laying hens M Sinclair,Poultry science,2024
Broiler physiological response to low phosphorus diets at different stages of production AO Omotoso,Poultry science,2022
Dietary encapsulated Bacillus subtilis and essential oil supplementation improves reproductive performance and hormone concentrations of broiler breeders during the late laying period Y.B. Chen,Livestock Science,2021
Effects of dietary calcium and available phosphorus levels and phytase supplementation on performance, bone mineral density, serum biochemical bone markers in aged white egg-laying hens. A Bello,Poultry Science,2020
Effects of dietary phosphorus and calcium levels and phytase supplementation on bone metabolism of egg-laying hens Abiodun Bello, et al,/,2018

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功能：

PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion.
基因功能参考文献：
1. A single nucleotide polymorphism in parathyroid hormone affected the eggshell percentage and breaking strength, and it may be associated with the variation of serum calcium and PTH level. PMID: 20852100
亚细胞定位：

Secreted.
蛋白家族：

Parathyroid hormone family
数据库链接：

KEGG: gga:396436

STRING: 9031.ENSGALP00000008579

UniGene: Gga.78

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.