Sheep Vasopressin-neurophysin 2-copeptin(AVP) ELISA kit

Sheep Vasopressin-neurophysin 2-copeptin(AVP) ELISA kit

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中文名称：

绵羊加压素-神经垂体素运载蛋白-2-和肽素(AVP)酶联免疫试剂盒
货号：

CSB-EL002466SH
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO绵羊加压素-神经垂体素运载蛋白-2-和肽素（AVP）定量检测专用酶联免疫试剂盒（货号：CSB-EL002466SH），基于双抗体夹心法原理开发，适用于血清、血浆及组织匀浆样本的精准检测。AVP作为神经内分泌调节肽，通过与受体结合参与水盐代谢平衡、血压调控及应激反应等重要生理过程，其水平变化常被用于评估神经内分泌功能状态。该试剂盒检测灵敏度达1.56 pg/mL，线性检测范围为1.56-100 pg/mL，采用预包被特异性抗体的96孔板实现目标蛋白的高效捕获，通过酶标二抗显色系统完成定量分析。实验全程无需复杂样本前处理，配套标准品和反应试剂可实现批量样本的高通量检测，适用于科研领域探究绵羊心血管系统、肾脏功能或应激生理机制的相关研究，特别是在动物模型的水代谢异常、抗利尿激素相关调控机制等方向具有重要应用价值，为神经内分泌领域的分子机制研究提供可靠工具。
别名：

ADH, ARVP, AVP-NPII, AVRP, VP, antidiuretic hormone|arginine vasopressin-neurophysin II|neurohypophyseal|vasopressin-neurophysin II-copeptin
缩写：

AVP
种属：

Ovis aries (Sheep)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-94
1:2	Average %	101
	Range %	95-105
1:4	Average %	92
	Range %	87-95
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of sheep AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	86-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.500	2.345	2.423	2.296
50	1.922	1.889	1.906	1.779
25	1.224	1.253	1.239	1.112
12.5	0.755	0.776	0.766	0.639
6.25	0.445	0.464	0.455	0.328
3.12	0.316	0.303	0.310	0.183
1.56	0.216	0.211	0.214	0.087
0	0.128	0.126	0.127

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.