Dog arginine vasopressin (AVP) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬抗利尿激素/血管加压素/精氨酸加压素 (AVP)酶联免疫试剂盒（CSB-EL002466DO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的AVP含量。AVP即精氨酸加压素，在人体水盐平衡、血压调节、垂体激素释放等生理过程中发挥重要作用。其研究机制主要围绕AVP受体展开，探索通过调节受体活性来干预相关疾病，如心力衰竭、多尿症等，为开发针对性药物提供方向。试剂盒检测范围为0.78 pg/mL-50 pg/mL，适用于科研领域探究犬类内分泌系统功能、心血管疾病模型、脱水或水肿相关代谢研究，以及药理学实验中药物对体液调节通路的影响评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

AVP
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma
检测范围：

0.78 pg/mL-50 pg/mL
灵敏度：

0.2 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	96
	Range %	92-101
1:4	Average %	86
	Range %	81-92
1:8	Average %	90
	Range %	84-97

回收率：

The recovery of dog AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	107	103-111

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.