Mouse antidiuretic hormone/vasopressin/arginine vasopressin,ADH/VP/AVP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CSB-E09272m小鼠抗利尿激素/血管加压素/精氨酸加压素(ADH/VP/AVP)酶联免疫试剂盒采用双抗体夹心法原理进行定量检测，适用于血清、血浆及细胞培养液上清样本中ADH/VP/AVP的精准分析。该靶标激素由下丘脑合成并通过神经垂体释放，在维持体液渗透压、血压调节及肾脏水重吸收等生理过程中起关键作用，常用于内分泌机制、水盐代谢研究及高血压或尿崩症动物模型的科研分析。本试剂盒检测灵敏度覆盖1.25至80皮克/毫升线性范围，通过高特异性配对抗体实现目标分子的双位点捕获与信号放大，实验流程包含预包被板、标准品、检测抗体及显色系统等完整组件，支持96孔板规格操作。适用于实验室对神经内分泌调节机制、病理生理模型中的激素动态变化以及药物干预效应等科研场景的定量研究，具备操作便捷性和结果重复性优势，为相关基础研究提供可靠工具。
别名：

ADH, ARVP, AVP-NPII, AVRP, VP, antidiuretic hormone|arginine vasopressin-neurophysin II|neurohypophyseal|vasopressin-neurophysin II-copeptin
缩写：

AVP
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates
检测范围：

1.25 pg/mL-80 pg/mL
灵敏度：

0.31 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADH/VP/AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	89
	Range %	84-95
1:4	Average %	87
	Range %	82-96
1:8	Average %	96
	Range %	90-104

回收率：

The recovery of mouse ADH/VP/AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	83-94
EDTA plasma (n=4)	102	98-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.