Rat platelet-derived growth factor-BB,PDGF-BB ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠血小板衍生生长因子BB(PDGF-BB)酶联免疫试剂盒（CSB-E08924r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PDGF-BB含量。PDGF-BB属于血小板衍生生长因子，在细胞的生长、增殖和分化中发挥重要作用。其异常表达与肿瘤血管生成、纤维化疾病等多种病理过程相关。研究通过抑制其信号通路，阻断细胞增殖与迁移，有望为相关疾病治疗提供新策略。试剂盒检测范围为39.063 pg/mL-2500 pg/mL，该产品适用于基础科研领域，如细胞模型中生长因子调控机制研究、动物疾病模型中PDGF - BB动态变化的定量分析，以及组织微环境修复相关实验的分子水平检测，为探索心血管疾病、组织再生及病理损伤等研究方向提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PDGF-BB
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

39.063 pg/mL-2500 pg/mL
灵敏度：

9.77 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	101-107
1:2	Average %	97
	Range %	94-100
1:4	Average %	88
	Range %	83-90
1:8	Average %	89
	Range %	84-93

回收率：

The recovery of rat PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	101-109
EDTA plasma (n=4)	110	107-113

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.