Mouse platelet-derived growth factor-BB,PDGF-BB ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠血小板衍生生长因子BB(PDGF-BB)酶联免疫试剂盒（CSB-E08925m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-BB含量。PDGF-BB 是血小板衍生生长因子异构体。其背景在于它在细胞生长、增殖等生理过程有关键作用。研究机制主要围绕其与受体结合，激活下游信号通路，在肿瘤血管生成、纤维化疾病发展等病理过程有重要影响，也是药物研发热门靶点。试剂盒检测范围为0.18 ng/mL-12 ng/mL，科研人员可将其应用于小鼠模型中的炎症反应研究、血管生成机制探索或组织再生相关实验，也可用于评估药物干预或基因修饰对 PDGF-BB 表达的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PDGF-BB
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.18 ng/mL-12 ng/mL
灵敏度：

0.047 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-88
1:2	Average %	94
	Range %	89-98
1:4	Average %	104
	Range %	97-109
1:8	Average %	99
	Range %	93-105

回收率：

The recovery of mouse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	89	82-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.