Horse platelet-derived growth factor-BB(PDGF-BB) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO马源血小板衍生生长因子BB（PDGF-BB）酶联免疫检测试剂盒（货号：CSB-EQ027241HO），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中PDGF-BB的浓度。PDGF-BB是一种重要的细胞生长因子，参与细胞增殖、血管生成及组织修复等生理过程，其水平变化与炎症反应、纤维化疾病及肿瘤微环境调控等研究领域密切相关。本试剂盒检测范围为1.56-100 ng/mL，可精准覆盖常见生物样本中PDGF-BB的表达量，实验操作中需将样本与生物素标记检测抗体及辣根过氧化物酶（HRP）标记链霉亲和素进行孵育，通过显色反应实现目标蛋白定量分析。适用于科研场景中探究心血管疾病模型、创伤修复机制或干细胞分化调控等相关课题，为体外实验中PDGF-BB的功能研究提供可靠的数据支持，需注意实验过程中需自备酶标仪、洗板机等基础设备。
缩写：

PDGF-BB
种属：

Equus caballus (Horse)
样本类型：

serum, plasma,tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	94
	Range %	91-99
1:4	Average %	84
	Range %	81-87
1:8	Average %	83
	Range %	80-86

回收率：

The recovery of horse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	101-109
EDTA plasma (n=4)	94	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.