Rat plasmin-antiplasmin complex,PAP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠血清或组织匀浆样本中纤溶酶抗纤溶酶复合物（PAP）的酶联免疫试剂盒（货号CSB-E08435r），采用双抗体夹心法原理，可精准测定7.8-500 ng/mL浓度范围的PAP水平。PAP是纤溶酶与其抑制剂α2-抗纤溶酶形成的稳定复合物，其浓度反映体内纤维蛋白溶解系统的活性状态，在血栓性疾病、纤溶亢进等病理机制研究中具有重要生物学意义。试剂盒包含预包被特异性抗体的96孔板及标准品、检测抗体、HRP标记链霉亲和素等核心组分，通过生物素-亲和素信号放大系统实现高灵敏度检测，适用于心血管疾病模型研究、凝血-纤溶平衡机制探索或药物干预效果评估等科研领域。实验操作简便，样本兼容性强，支持血清直接检测及组织匀浆离心上清液分析，可为实验室提供稳定可靠的PAP定量数据支持。
别名：

N/A
缩写：

PAP
种属：

Rattus norvegicus (Rat)
样本类型：

serum, tissue homogenates
检测范围：

7.8 ng/mL-500 ng/mL
灵敏度：

1.95 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-103
1:2	Average %	98
	Range %	91-105
1:4	Average %	99
	Range %	94-106
1:8	Average %	105
	Range %	100-110

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.