Mouse plasmin-antiplasmin complex,PAP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠纤溶酶抗纤溶酶复合物(PAP)酶联免疫检测试剂盒（货号CSB-E08436m），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的PAP水平，检测灵敏度为3.12 pg/mL，线性范围覆盖3.12-200 pg/mL。PAP是纤溶系统激活的关键生物标志物，由纤溶酶与α2-抗纤溶酶特异性结合形成，其浓度变化可反映纤溶活性状态，常用于研究凝血与纤溶平衡机制。试剂盒包含预包被抗体的96孔板、标准品及检测所需试剂，操作流程约需4.5小时完成，适用于体外实验环境下检测小鼠模型中与血栓形成、溶栓治疗或炎症反应相关的PAP动态变化，例如在血栓性疾病模型评估、抗凝药物作用机制研究或纤溶系统调控实验中定量分析样本生物标志物水平。该试剂盒提供高特异性抗体对，确保检测结果准确性，为科研人员研究纤溶系统功能及相关病理生理机制提供可靠工具。
别名：

N/A
缩写：

PAP
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	87
	Range %	83-94
1:10	Average %	97
	Range %	92-102
1:20	Average %	105
	Range %	98-111
1:40	Average %	93
	Range %	87-99

回收率：

The recovery of mouse PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	101	95-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.