Human plasmin-antiplasmin complex,PAP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人纤溶酶抗纤溶酶复合物（PAP）是纤溶系统调控的关键标志物，由纤溶酶与α2-抗纤溶酶结合形成，其浓度变化可反映纤溶活性状态，在血栓性疾病、纤维蛋白溶解异常及炎症反应等病理机制研究中具有重要价值。本试剂盒（货号：CSB-E08434h）采用双抗体夹心法原理，通过特异性捕获和检测抗体实现对PAP的高灵敏度定量分析，检测范围覆盖0.156 ng/mL至10 ng/mL，适用于血清、血浆、细胞培养上清及尿液样本的科研检测。该产品可为研究血栓形成与溶解的动态平衡、评估抗凝药物作用机制或探索纤溶相关疾病的生物标志物提供可靠工具，例如在心血管疾病模型、肿瘤微环境分析或炎症相关实验中进行样本内源性PAP水平的精准测定。试剂盒操作便捷，配套标准品及优化缓冲体系可确保实验重复性，满足体外实验中对微量复合物的定量需求，为生命科学基础研究提供高效解决方案。
别名：

N/A
缩写：

PAP
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, urine
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	80-92
1:200	Average %	100
	Range %	91-108
1:400	Average %	100
	Range %	92-109
1:800	Average %	92
	Range %	86-100

回收率：

The recovery of human PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-101
EDTA plasma (n=4)	93	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.