Rat oxytocin,OT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠血清或血浆中催产素（OT）含量的竞争法ELISA试剂盒，采用预包被抗体的酶标板进行检测。催产素作为神经肽激素，在调节生殖行为、母性关怀及社会联结等生理过程中发挥重要作用。试剂盒检测灵敏度达7.5 pg/mL，线性范围为7.5-600 pg/mL，可精准检测样本中OT的动态变化。实验原理基于样本中的OT与标记物竞争结合固相抗体，通过显色反应强度与目标物浓度成反比的特性实现定量分析，适用于神经内分泌研究、行为学实验及压力反应机制等科研领域。该试剂盒操作流程包含样本预处理、孵育反应及酶标仪检测（建议450nm波长读数）等标准化步骤，配套提供完整试剂组分和操作说明书。科研人员可通过该产品深入探究OT在焦虑调控、分娩启动或社会互动中的分子机制，为神经生物学和内分泌学研究提供可靠工具。
缩写：

OT
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma.
检测范围：

7.5 pg/mL-600 pg/mL
灵敏度：

9.375 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	95-102
1:2	Average %	93
	Range %	90-96
1:4	Average %	98
	Range %	95-102
1:8	Average %	101
	Range %	98-104

回收率：

The recovery of rat OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	92	89-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.