Human oxytocin,OT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人催产素（OT）酶联免疫试剂盒（货号：CSB-E08994h），采用双抗体夹心法原理进行定量检测，适用于分析血清、血浆及组织匀浆样本中的OT浓度。催产素是一种由下丘脑合成的神经肽激素，在分娩刺激、哺乳调节及社会行为中发挥重要作用，其水平变化与母婴健康、情绪调控及神经内分泌研究密切相关。本试剂盒检测范围为8-400 μIU/mL，通过包被特异性抗体实现高灵敏度检测，可精准识别样本中的OT分子，适用于基础科研中探索神经生物学机制、行为学关联性分析或母婴健康相关研究。试剂盒操作便捷，兼容常规实验室设备，配套标准品及预包被酶标板，实验流程优化后可在4.5小时内完成检测，满足多样本量需求。该产品为神经科学、心理学及内分泌学领域的研究者提供可靠工具，用于定量分析生物样本中OT的动态变化，支持探索其在生理调控及疾病模型中的潜在作用。
别名：

N/A
缩写：

OT
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

8 μIU/mL-400 μIU/mL
灵敏度：

10 μIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-96
1:2	Average %	95
	Range %	91-99
1:4	Average %	98
	Range %	92-103
1:8	Average %	93
	Range %	87-98

回收率：

The recovery of human OT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	96	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.