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Pig oxytocin,OT ELISA Kit

  • 中文名称:
    猪催产素(OT)酶联免疫试剂盒
  • 货号:
    CSB-E12062p
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

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  • 产品描述:
    CUSABIO猪催产素(OT)酶联免疫检测试剂盒(货号:CSB-E12062p),基于竞争法原理实现定量分析,适用于血清、血浆及组织匀浆样本中OT含量的精准检测。催产素作为神经垂体分泌的九肽激素,在生殖调控、母婴行为建立及社会关系中发挥关键作用,其水平变化与分娩启动、乳汁分泌及应激反应等生理过程密切相关。试剂盒采用高灵敏度检测体系,覆盖2.5-100 pg/mL线性范围,可有效识别样本中微量OT分子,为生殖生理学、神经内分泌学及动物行为学领域的基础研究提供可靠工具。科研人员可通过该试剂盒探究母猪分娩机制、泌乳调控规律,或分析OT在社交行为及环境适应中的分子调控网络,尤其适用于比较医学模型构建及畜牧养殖相关激素代谢研究。
  • 别名:
    MGC126890, MGC126892, OT, OT-NPI, neurophysin I|oxytocin, prepro- (neurophysin I)|oxytocin-neurophysin I|oxytocin-neurophysin I, preproprotein
  • 缩写:
    OT
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    2.5 pg/mL-100 pg/mL
  • 灵敏度:
    2.5 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig oxytocin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 91-105  
    1:2 Average % 95  
    Range % 90-100  
    1:4 Average % 92  
    Range % 87-99  
    1:8 Average % 95  
    Range % 90-101  
  • 回收率:
    The recovery of pig oxytocin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 90-96  
    EDTA plasma (n=4) 96 91-103  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    0 2.451 2.428 2.440    
    2.5 1.052 1.029 1.041    
    7.5 0.551 0.536 0.544    
    23 0.381 0.392 0.387    
    50 0.275 0.262 0.269    
    100 0.144 0.142 0.143    
  • 数据处理:
  • 货期:
    3-5 working days

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