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中文名称:大鼠多巴胺(DA)酶联免疫试剂盒
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货号:CSB-E08660r
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规格:96T/48T
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价格:¥3900/¥2500
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其他:
产品详情
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产品描述:本试剂盒(货号:CSB-E08660r)采用双抗体夹心法定量检测大鼠血清、血浆或组织匀浆中的多巴胺(DA)。DA作为中枢神经系统关键神经递质,在运动调控、奖赏机制及神经精神疾病研究中具有重要价值。试剂盒通过高特异性抗体对捕获样本中的DA分子,检测灵敏度达0.156 ng/mL,线性检测区间覆盖0.156至10 ng/mL,满足低浓度样本的精准分析需求。该产品适用于神经生物学、药理学等科研领域,可探究帕金森模型动物DA水平变化、精神疾病相关神经递质动态,或评估药物干预对DA代谢通路的影响。实验操作采用标准96孔板模式,兼容常规酶标仪检测,提供预包被板、标准品及检测所需全套试剂,具备良好的批间稳定性和重复性,为研究多巴胺能系统功能及相关疾病机制提供可靠的实验工具。
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别名:N/A
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缩写:DA
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, tissue homogenates
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检测范围:0.156 ng/mL-10 ng/mL
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灵敏度:0.039 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Neuroscience
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 86 Range % 84-96 1:10 Average % 95 Range % 91-100 1:20 Average % 84 Range % 81-88 1:40 Average % 92 Range % 88-96 -
回收率:
The recovery of rat DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-95 EDTA plasma (n=4) 96 94-98 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.174 2.280 2.227 2.076 5 1.784 1.789 1.787 1.636 2.5 1.250 1.276 1.263 1.112 1.25 0.841 0.875 0.858 0.707 0.625 0.435 0.465 0.450 0.299 0.312 0.287 0.308 0.298 0.147 0.156 0.208 0.223 0.216 0.065 0 0.152 0.150 0.151 -
数据处理:
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货期:3-5 working days
引用文献
- Study of Neuroinflammation and Oxidative Stress Induced by Energy Drinks Consumption A Dose-Dependent Study S Sherif, N Gamal, O Abdelnaby, A Hamza,Azerbaijan Medical Journal,/
- Neuroprotective Effects of Bryophyllum pinnatum against Ketamine-Induced Neurotoxicity in Wistar Rats: Neurochemical, Oxidative Stress, and Histological Investigation PO Uahomo, JC Isirima,International Neuropsychiatric Disease Journal,2024
- Neuroprotective effect of Lannea egregia Alkaloid-rich Leaf Extracts in Streptozotocin-induced Diabetic Rats BO Ajiboye, BE Ekundayo, AW Salami, AO Osukoya,Toxicology Reports,2024
- Boosting Physical Performance in SD Rats through Brain-Targeted Delivery of Caffeine-Loaded Transferrin Liposomes H Yun,Heliyon,2024
- The analgesic effects of insulin and its disorders in streptozotocin‐induced short‐term diabetes AM Basatinya,Physiological reports,2024
- A Complex Interplay of DJ-1, LRRK2, and Nrf2 in the Regulation of Mitochondrial Function in Cypermethrin-Induced Parkinsonism A Sarkar,Molecular neurobiology,2023
- Chitosan-coated nanostructured lipid carriers for effective brain delivery of Tanshinone IIA in Parkinson’s disease: interplay between nuclear factor-kappa β and cathepsin B DM Hassan,Drug delivery and translational research,2023
- Targeting α7-nAChR by galantamine mitigates reserpine-induced fibromyalgia-like symptoms in rats: involvement of cAMP/PKA, PI3K/AKT, and M1/M2 microglia … AA Atta,European journal of pharmacology,2023
- Reactive astrocytes targeting with oral vitamin A: Efficient neuronal regeneration for Parkinson's disease treatment and reversal of associated liver fibrosis NS El‐Mezayen,CNS neuroscience & therapeutics,2023
- Gut microbiota-SCFAs-brain axis associated with the antidepressant activity of berberine in CUMS rats MN Huang,Journal of affective disorders,2023
- p-CREB and p-DARPP-32 orchestrating the modulatory role of cAMP/PKA signaling pathway enhanced by Roflumilast in rotenone-induced Parkinson's disease in rats RM Essam,Chemico-biological interactions,2023
- Cilostazol Alleviates NLRP3 Inflammasome–Induced Allodynia/Hyperalgesia in Murine Cerebral Cortex Following Transient Ischemia: Focus on TRPA1/Glutamate and Akt/Dopamine/BDNF/Nrf2 Trajectories OS Zaki,Molecular Neurobiology,2022
- Alhagi maurorum Ethanolic Extract Rescues Hepato-Neurotoxicity and Neurobehavioral Alterations Induced by Lead in Rats via Abrogating Oxidative Stress and the Caspase-3-Dependent Apoptotic Pathway TM Saber,Antioxidants,2022
- Anti-aging trait of whey protein against brain damage of senile rats ME El-Beeh,Anti-aging trait of whey protein against brain damage of senile rats,2022
- Design and synthesis of benzo[d]thiazol-2-yl-methyl-4-(substituted)-piperazine-1-carbothioamide as novel neuronal nitric oxide inhibitors and evaluation of their neuroprotecting effect in 6-OHDA-induced unilateral lesioned rat model of Parkinson's disease S Agrawal,Biomedicine & Pharmacotherapy,2022
- Neuroprotective effect of peanut against oxidative stress in streptozotocin-induced diabetic rats NH Mohamed,Egyptian Journal of Basic and Applied Sciences,2022
- Cilostazol Alleviates NLRP3 Inflammasome–Induced Allodynia/Hyperalgesia in Murine Cerebral Cortex Following Transient Ischemia: Focus on TRPA1/Glutamate and Akt/Dopamine/BDNF/Nrf2 Trajectories OS Zaki,Molecular neurobiology,2022
- Molecular evidence of the amelioration of toluene induced encephalopathy by human breast milk mesenchymal stem cells OIA Hamid,Scientific reports,2022
- Neurotoxic Effect of Fipronil in Male Wistar Rats: Ameliorative Effect of L-Arginine and L-Carnitine YK Mahmoud,Biology,2021
- Altered hippocampal function and cytokine levels in a rat model of Gulf War illness Joyce G. Rohan,Life Sciences,2021
- Targeting ROS-Dependent AKT/GSK-3β/NF-κB and DJ-1/Nrf2 Pathways by Dapagliflozin Attenuates Neuronal Injury and Motor Dysfunction in Rotenone-Induced Parkinson’s Disease Rat Model Marwa M. Safar,ACS Chemical Neuroscience,2021
- Influence of progestational stress on BDNF and NMDARs in the hippocampus of male offspring and amelioration by Chaihu Shugan San Xin-xing Wang,Biomedicine & Pharmacotherapy,2021
- Unique Novel role of Adropin in the gastric Ulcer in a rotenone-induced Rat model of Parkinson's disease RE Abo El gheit,ACS Chemical Neuroscience,2020
- Effect of Solanum macrocarpon Linn leaf aqueous extract on the brain of an alloxan-induced rat model of diabetes MA Okesola,J Int Med Res,2020
- Saponin extract of Baihe-Zhimu Tang ameliorates depression in chronic mild stress rats D Jia,Journal of Functional Foods,2020
- Mechanism of the neuroprotective effect of GLP-1 in a rat model of Parkinson's with pre-existing diabetes Elbassuoni E A, et al,Neurochemistry International,2019
- Correlation between angiotensin 1-7-mediated Mas receptor expression with motor improvement, activated STAT3/SOCS3 cascade, and suppressed HMGB-1/RAGE/NF-κB signaling in 6-hydroxydopamine hemiparkinsonian rats Mostafa A.Rabie, et al,Biochemical Pharmacology,2019
- Protective effects of betanin against paracetamol and diclofenac induced neurotoxicity and endocrine disruption in rats Motawi TK, et al,Biomarkers,2019
- Comparing the effects of progressive and mild intensity treadmill running protocols on neuroprotection of parkinsonian rats Mohammadi ZF,Life Sciences,2019
- Sitagliptin and Liraglutide Modulate L-dopa Effect and Attenuate Dyskinetic Movements in Rotenone-Lesioned Rats Badawi GA, et al,Neurotoxicity Research,2019
- A pharmacological composition for induction of a reversible torpor-like state and hypothermia in rats Zakharova NM, et al,Life Sciences,2019
- Social defeat stress before pregnancy induces depressive-like behaviours and cognitive deficits in adult male offspring: correlation with neurobiological changes Wei S.et al,BMC Neuroscience,2018
- INCREASED LOCOMOTOR ACTIVITY IN ESTROGEN-\TREATED OVARIECTOMIZED RATS IS ASSOCIATED WITH NUCLEUS ACCUMBENS DOPAMINE AND IS NOT REDUCED BY DIETARY SODIUM DEPRIVATION Enith Espinosa.et al,INTEGR ZOOL,2018
- Neuroprotective effect of IDPU (1-(7-imino-3-propyl-2,3-dihydrothiazolo [4,5-d]pyrimidin-6(7H)-yl)urea) in 6-OHDA induced rodent model of hemiparkinson’s disease NamrataKumari.et al,Neuroscience Letters,2018
- A urinary metabolomics (GC-MS) strategy to evaluate the antidepressant-like effect of chlorogenic acid in adrenocorticotropic hormone-treated rats Le Zhao.et al, RSC Adv,2018
- Effect of Yulangsan Polysaccharide on the Reinstatement of Morphine-Induced Conditioned Place Preference in Sprague?Dawley Rats Chen C.et al,Neurochem Res,2018
- Angiotensin 1-7 ameliorates 6-hydroxydopamine lesions in hemiparkinsonian rats through activation of MAS receptor/PI3K/Akt/BDNF pathway and inhibition Rabie, Mostafa A.et al,Biochemical pharmacology,2018
- Impact of anger emotional stress before pregnancy on adult male offspring Wei S.et al,Oncotarget,2017
- Antidepressant-like effect of lipid extract of Channa striatus in postpartum model of depression in rats Abdul Shukkoor MS.et al,Evid Based Complement Alternat Med,2016
- Forced Exercise in Combination with 1, 25-dihydroxyvitamin D3 Before Induction of 6-hydroxydopamine Protects Dopaminergic Neurons in Rats Model of Parkinson’s Disease Fatemeh Mirfakhrai .et al,Zahedan Journal of Research in Medical Sciences,2017
- The role of apitoxin in alleviating propionic acid-induced neurobehavioral impairments in rat pups: The expression pattern of Reelin gene Daghestani MH.et al,Biomed Pharmacother.,2017
- Neurotoxic effects of silver nanoparticles and the protective role of rutin Ahmed MM.et al,Biomed Pharmacothe,2017
- Sitagliptin and liraglutide reversed nigrostriatal degeneration of rodent brain in rotenone-induced Parkinson’s disease Badawi GA.et al,Inflammopharmacology.,2017
- A novel GLP-1/GIP dual receptor agonist protects from 6-OHDA lesion in a rat model of Parkinson's disease Jalewa J.et al,Neuropharmacology.,2017
- Modulatory effects of resveratrol on endoplasmic reticulum stress-associated apoptosis and oxido-inflammatory markers in a rat model of rotenone-induced Parkinson's disease Gaballah HH.et al,Chem Biol Interact.,2016
- Comparative protective effects of royal jelly and cod liver oil against neurotoxic impact of tartrazine on male rat pups brain Mohamed AA. et al,Acta Histochem,2015
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最新研究进展:DA是多巴胺的简称,是一种重要的神经递质。近年来,研究人员发现DA参与了多种生理和病理过程,如运动功能、认知能力、代谢性疾病、精神疾病等。同时,DA与多种神经系统疾病的发病机制密切相关,如帕金森病、精神分裂症等。
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