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Rat dopamine,DA ELISA Kit

  • 中文名称:
    大鼠多巴胺(DA)酶联免疫试剂盒
  • 货号:
    CSB-E08660r
  • 规格:
    96T/48T
  • 价格:
    ¥3900/¥2500
  • 其他:

产品详情

  • 产品描述:
    本试剂盒(货号:CSB-E08660r)采用双抗体夹心法定量检测大鼠血清、血浆或组织匀浆中的多巴胺(DA)。DA作为中枢神经系统关键神经递质,在运动调控、奖赏机制及神经精神疾病研究中具有重要价值。试剂盒通过高特异性抗体对捕获样本中的DA分子,检测灵敏度达0.156 ng/mL,线性检测区间覆盖0.156至10 ng/mL,满足低浓度样本的精准分析需求。该产品适用于神经生物学、药理学等科研领域,可探究帕金森模型动物DA水平变化、精神疾病相关神经递质动态,或评估药物干预对DA代谢通路的影响。实验操作采用标准96孔板模式,兼容常规酶标仪检测,提供预包被板、标准品及检测所需全套试剂,具备良好的批间稳定性和重复性,为研究多巴胺能系统功能及相关疾病机制提供可靠的实验工具。
  • 别名:
    N/A
  • 缩写:
    DA
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.156 ng/mL-10 ng/mL
  • 灵敏度:
    0.039 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 86  
    Range % 84-96  
    1:10 Average % 95  
    Range % 91-100  
    1:20 Average % 84  
    Range % 81-88  
    1:40 Average % 92  
    Range % 88-96  
  • 回收率:
    The recovery of rat DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-95  
    EDTA plasma (n=4) 96 94-98  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.174 2.280 2.227 2.076  
    5 1.784 1.789 1.787 1.636  
    2.5 1.250 1.276 1.263 1.112  
    1.25 0.841 0.875 0.858 0.707  
    0.625 0.435 0.465 0.450 0.299  
    0.312 0.287 0.308 0.298 0.147  
    0.156 0.208 0.223 0.216 0.065  
    0 0.152 0.150 0.151    
  • 数据处理:
  • 货期:
    3-5 working days

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靶点详情

  • 最新研究进展:
    DA是多巴胺的简称,是一种重要的神经递质。近年来,研究人员发现DA参与了多种生理和病理过程,如运动功能、认知能力、代谢性疾病、精神疾病等。同时,DA与多种神经系统疾病的发病机制密切相关,如帕金森病、精神分裂症等。