Human dopamine,DA ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人多巴胺(DA)酶联免疫试剂盒（CSB-E06888h）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DA含量。DA即多巴胺，是一种重要神经递质。其在调节运动、情感、认知等生理过程中发挥关键作用，异常时会引发如帕金森病、精神分裂症等多种疾病。相关研究聚焦于其合成、释放、代谢机制及与受体相互作用，为开发针对性治疗药物提供方向。试剂盒检测范围为0.5 ng/mL-100 ng/mL，该产品支持多样本类型分析，便于研究人员探索不同生物基质中多巴胺的动态变化，如神经药理学研究中药物干预对神经递质释放的影响、疾病模型动物脑组织多巴胺代谢评估，或体外培养神经元/神经母细胞瘤细胞的多巴胺分泌水平监测。适用于神经生物学、行为学及分子机制研究中的多巴胺定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

DA
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.5 ng/mL-100 ng/mL
灵敏度：

0.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	90
	Range %	86-94
1:4	Average %	90
	Range %	86-94
1:8	Average %	102
	Range %	97-105

回收率：

The recovery of human DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-98
EDTA plasma (n=4)	94	89-98