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Fish dopamine(DA) ELISA kit

  • 中文名称:
    鱼多巴胺(DA)酶联免疫试剂盒
  • 货号:
    CSB-EQ027496FI
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

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  • 产品描述:
    CUSABIO鱼类多巴胺(Dopamine, DA)酶联免疫检测试剂盒(货号:CSB-EQ027496FI),适用于定量检测鱼类血清、血浆及组织匀浆样本中的DA含量。多巴胺作为重要的单胺类神经递质,在鱼类神经信号传递、应激调控及生理代谢过程中发挥关键作用,其水平变化可反映神经系统状态与环境适应能力。试剂盒采用竞争法检测原理,通过特异性抗体捕获样本中的DA分子,酶标抗原与游离DA竞争结合位点,经显色反应后通过标准曲线计算目标物浓度,检测灵敏度达62.5 pg/mL,线性范围为62.5-1000 pg/mL,可精准识别微量DA含量差异。该产品适用于鱼类神经生物学研究、水产养殖健康监测、环境污染物对水生生物神经系统的毒性评估等科研领域,特别在水体环境毒理学实验中,可通过检测鱼类血液或组织DA水平评估重金属、农药等污染物对中枢神经系统的影响。实验操作全程采用标准化预包被板,配套提供完整试剂体系,支持科研人员高效完成样本中DA的定量分析。
  • 缩写:
    DA
  • 种属:
    Fish
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    62.5 pg/mL-1000 pg/mL
  • 灵敏度:
    62.5 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 92-104  
    1:2 Average % 94  
    Range % 91-98  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 88  
    Range % 82-94  
  • 回收率:
    The recovery of fish DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 103 98-107  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.154 0.155 0.155    
    500 0.278 0.272 0.275    
    250 0.503 0.518 0.511    
    125 1.135 1.174 1.155    
    62.5 2.345 2.428 2.387    
  • 数据处理:
  • 货期:
    3-5 working days

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