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中文名称:鱼多巴胺(DA)酶联免疫试剂盒
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货号:CSB-EQ027496FI
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规格:96T/48T
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价格:¥4200/¥3000
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其他:
产品详情
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产品描述:CUSABIO鱼类多巴胺(Dopamine, DA)酶联免疫检测试剂盒(货号:CSB-EQ027496FI),适用于定量检测鱼类血清、血浆及组织匀浆样本中的DA含量。多巴胺作为重要的单胺类神经递质,在鱼类神经信号传递、应激调控及生理代谢过程中发挥关键作用,其水平变化可反映神经系统状态与环境适应能力。试剂盒采用竞争法检测原理,通过特异性抗体捕获样本中的DA分子,酶标抗原与游离DA竞争结合位点,经显色反应后通过标准曲线计算目标物浓度,检测灵敏度达62.5 pg/mL,线性范围为62.5-1000 pg/mL,可精准识别微量DA含量差异。该产品适用于鱼类神经生物学研究、水产养殖健康监测、环境污染物对水生生物神经系统的毒性评估等科研领域,特别在水体环境毒理学实验中,可通过检测鱼类血液或组织DA水平评估重金属、农药等污染物对中枢神经系统的影响。实验操作全程采用标准化预包被板,配套提供完整试剂体系,支持科研人员高效完成样本中DA的定量分析。
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缩写:DA
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种属:Fish
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样本类型:serum, plasma, tissue homogenates
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检测范围:62.5 pg/mL-1000 pg/mL
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灵敏度:62.5 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Neuroscience
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 92-104 1:2 Average % 94 Range % 91-98 1:4 Average % 102 Range % 98-106 1:8 Average % 88 Range % 82-94 -
回收率:
The recovery of fish DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 103 98-107 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 1000 0.154 0.155 0.155 500 0.278 0.272 0.275 250 0.503 0.518 0.511 125 1.135 1.174 1.155 62.5 2.345 2.428 2.387 -
数据处理:
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货期:3-5 working days
引用文献
- Integrated Analysis of Neuroendocrine and Neurotransmission Pathways Following Developmental Atrazine Exposure in Zebrafish SC Stradtman, JN Swihart, K Moore, IN Akoro,International Journal of Molecular Sciences,2024
- Neuroprotective effects of nanogold-based Ayurveda medicine Suvarna Bhasma against rotenone-induced Parkinson's-like model S Biswas,Journal of Ayurveda and integrative medicine,2023
- Differential Developmental Neurotoxicity and Tissue Uptake of the Per-and Polyfluoroalkyl Substance Alternatives, GenX and PFBS O Wasel,Environmental science & technology,2023
- Effects of two alternative plasticizers on the growth hormone-related endocrine system, neurodevelopment, and oxidative stress of zebrafish larvae K Yun,Environmental pollution,2023
- Low concentrations of the antidepressant venlafaxine affect courtship behaviour and alter serotonin and dopamine systems in zebrafish (Danio rerio) Y Tang,Aquatic toxicology,2023
- Potential Adverse Outcome Pathway of Oxidative Stress Induction and Neurodevelopmental Toxicity Mediated by Three Alkyl Organophosphate Flame Retardants in Zebrafish Larvae CH Lee,/,2024
- Different action mechanisms of low-and high-level quercetin in the brains of adult zebrafish (Danio rerio) X Wu,Ecotoxicology and Environmental Safety,2021
- Assessment of the Effects of Bisphenol A on Dopamine Synthesis and Blood Vessels in the Goldfish Brain Wang Q, et al,International Journal of Molecular Sciences,2019
- Venlafaxine plus melatonin ameliorate reserpine-induced depression-like behavior in zebrafish Ya-Qiu Tang, et al,Neurotoxicology and Teratology,2019
- Transcriptional analysis of renal dopamine-mediated Na+ homeostasis response to environmental salinity stress in Scatophagus argus Su M,BMC Genomics,2019
- Dopamine regulates renal osmoregulation during hyposaline stress via DRD1 in the spotted scat (Scatophagus argus) Maoliang Su.et al,Scientific Reports,2016
- Changes of physiological rhythms of N-methyl-d-aspartate receptors in the chum salmon Oncorhynchus keta: effect of seawater acclimation during the parr-smolt transformation Young Jae Choi.et al,Biological Rhythm Research,2016
- Ambient particulate air pollution and circulating antioxidant enzymes: A repeated-measure study in healthy adults in Beijing, China Shaowei Wu. et al,Environ Pollut,2015
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