Rat Pentosidine ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠戊糖素（Pentosidine）酶联免疫检测试剂盒（货号：CSB-E09416r），采用双抗体夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本中15.6 pmol/mL至1000 pmol/mL浓度范围内的戊糖素分析。戊糖素作为晚期糖基化终末产物（AGEs）的重要标志物，其异常积累与糖尿病并发症、组织纤维化及衰老相关病理过程密切相关，在代谢性疾病机制、氧化应激研究及抗衰老药物开发等科研领域具有重要价值。该试剂盒通过高特异性抗体对实现靶标捕获与检测，操作流程标准化，可在4小时内完成检测，提供稳定的实验重复性。其宽泛的检测范围可满足不同样本浓度需求，尤其适用于糖尿病模型大鼠的血清/血浆样本分析，或肝脏、肾脏等组织的匀浆液检测，为研究戊糖素在慢性疾病发展中的作用机制、评估干预措施效果提供可靠工具。本产品专为科研实验设计，不用于临床诊断或其他用途。
缩写：

Pentosidine
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pmol/mL-1000 pmol/mL
灵敏度：

3.9 pmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-89
1:2	Average %	89
	Range %	85-93
1:4	Average %	96
	Range %	92-100
1:8	Average %	92
	Range %	86-97

回收率：

The recovery of rat Pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	96-105
EDTA plasma (n=4)	90	85-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.