Human Pentosidine ELISA Kit

Human Pentosidine ELISA Kit

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中文名称：

人戊糖素(Pentosidine)酶联免疫试剂盒
货号：

CSB-E09415h
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人戊糖素(Pentosidine)酶联免疫试剂盒（CSB-E09415h）是基于双抗体夹心法的定量检测工具，用于检测血清、血浆、组织匀浆及尿液样本中的戊糖素浓度。戊糖素作为晚期糖基化终末产物（AGEs）的重要标志物，在糖尿病并发症、慢性肾病及骨关节退行性病变等病理过程中具有重要研究价值，其积累水平与氧化应激和代谢紊乱密切相关。本试剂盒检测范围覆盖31.25 pmol/mL至2000 pmol/mL，可精准检测生物样本中宽浓度范围的戊糖素含量。实验采用预包被特异性抗体的微孔板，通过两步孵育结合样本中的目标蛋白，配合高灵敏度显色系统实现定量分析，操作流程标准化且重复性良好。该产品适用于基础医学研究、疾病机制探索、药物干预效果评估等科研场景，为代谢性疾病、衰老相关疾病及骨关节健康研究提供可靠的生物标志物检测方案。
缩写：

Pentosidine
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, urine
检测范围：

31.25 pmol/mL-2000 pmol/mL
灵敏度：

7.81 pmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	81-102
1:2	Average %	95
	Range %	84-105
1:4	Average %	104
	Range %	91-111
1:8	Average %	95
	Range %	80-103

回收率：

The recovery of human Pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-105
EDTA plasma (n=4)	94	88-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/ml	OD1	OD2	Average	Corrected
2000	2.677	2.697	2.687	2.537
1000	1.843	1.864	1.854	1.704
500	1.163	1.153	1.158	1.008
250	0.742	0.754	0.748	0.598
125	0.447	0.461	0.454	0.304
62.5	0.347	0.359	0.353	0.203
31.25	0.268	0.262	0.265	0.115
0	0.154	0.146	0.150

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Serum Pentosidine in Relation to Obesity in Patients with Type 2 Diabetes and Healthy Controls S Baumann, L Sewing, C Traechslin,Calcified Tissue International,2025
Serum Advanced Glycation End Products and Their Soluble Receptor as New Biomarkers in Systemic Lupus Erythematosus I Carrión,Biomedicines,2024
Effect of Vitamin D Receptor Activation on the AGE/RAGE System and Myeloperoxidase in Chronic Kidney Disease Patients Torino C,Oxidative medicine and cellular longevity,2023
The Burden of Impaired Serum Albumin Antioxidant Properties and Glyco-Oxidation in Coronary Heart Disease Patients with and without Type 2 Diabetes Mellitus F Piarulli,Antioxidants,2022
BONE HISTOMORPHOMETRY IN YOUNG PATIENTS WITH TYPE 2 DIABETES IS AFFECTED BY DISEASE CONTROL AND CHRONIC COMPLICATIONS Andrade V F C, et al,The Journal of Clinical Endocrinology & Metabolism,2019
A next generation setup for pre-fractionation of non-denatured proteins reveals diverse albumin proteoforms each carrying several post-translational modifications Rhode H, et al,Scientific Reports,2019
Increased level of advanced glycation end-products in renal transplant patients is associated with decreased measured GFR and grafted kidney function. Shahbazian, et al,Journal of Nephropathology,2019
The effects of n-3 long-chain polyunsaturated fatty acid supplementation on AGEs and sRAGE in type 2 diabetes mellitus Kurt A.et al,J Physiol Biochem.,2016
Effects of ginger on serum glucose, advanced glycation end products, and inflammation in peritoneal dialysis patients Imani H.et al,Nutrition.,2015
Pomegranate (Punicagranatum) juice decreases lipid peroxidation, but has no effect on plasma advanced glycated end-products in adults with type 2 Sohrab G. et al,Food Nutr Res,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.