Mouse Pentosidine ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠戊糖素（Pentosidine）酶联免疫检测试剂盒（货号CSB-E09417m），采用双抗体夹心法原理实现定量检测，适用于血清、血浆、细胞培养上清液及组织匀浆等多种生物样本的科研分析。戊糖素作为晚期糖基化终末产物（AGEs）的典型标志物，在糖尿病并发症、氧化应激诱导的组织损伤等病理过程中具有重要研究价值。试剂盒检测范围为31.25-2000 pg/mL，具备高灵敏度和宽动态范围，可精准检测样本中痕量目标物。实验操作通过预包被特异性抗体的微孔板捕获目标蛋白，结合HRP标记检测抗体形成复合物，最终通过显色反应进行定量分析。该试剂盒广泛应用于糖尿病模型研究、衰老相关氧化应激机制探索、代谢性疾病中蛋白质修饰分析等科研领域，为体外实验提供稳定可靠的检测工具。
缩写：

Pentosidine
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	91-103
1:200	Average %	85
	Range %	80-90
1:400	Average %	93
	Range %	89-98
1:800	Average %	99
	Range %	94-105

回收率：

The recovery of mouse pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.