Rat Folic acid,FA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠叶酸（FA）酶联免疫检测试剂盒（货号：CSB-E08757r），采用竞争法原理实现定量分析，适用于血清、血浆样本中叶酸含量的精确测定。叶酸作为B族维生素的重要组成部分，在DNA合成、细胞增殖及氨基酸代谢等生理过程中发挥关键作用，其水平检测可为营养代谢研究和生理机制探索提供重要依据。该试剂盒检测范围为19.5-5000 pg/mL，最低检测限为19.5 pg/mL，具备高灵敏度和宽线性范围特性，可满足微量样本的检测需求。实验操作基于预包被的FA-BSA结合物与样本中游离叶酸竞争结合特异性抗体的原理，通过显色反应实现目标物定量分析，全程操作标准化且重复性良好。本试剂盒适用于科研领域中对大鼠叶酸代谢相关研究，包括膳食营养评估、叶酸缺乏模型构建、药物干预效果分析等基础实验，为探索叶酸在生长发育、造血功能及神经系统中的作用机制提供可靠工具。
别名：

N/A
缩写：

FA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

19.5 pg/mL-5000 pg/mL
灵敏度：

19.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat folic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-104
1:2	Average %	92
	Range %	83-99
1:4	Average %	96
	Range %	87-98
1:8	Average %	94
	Range %	80-98

回收率：

The recovery of rat folic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	86-102
EDTA plasma (n=4)	94	88-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究进展：

叶酸（Folic acid）是一种维生素B族成员，参与人体DNA、RNA的合成和修复，对于细胞生长和分裂非常重要。最新的研究表明，叶酸在人体内具有多种生理功能，包括对于心血管健康和神经系统健康的影响，以及对于出生缺陷和某些类型的癌症的预防作用。