Mouse Folic acid,FA ELISA Kit

产品详情

产品描述：

CUSABIO小鼠叶酸（FA）酶联免疫检测试剂盒，采用竞争法定量分析血清、血浆样本中叶酸含量，检测范围覆盖0.195 ng/mL至50 ng/mL，灵敏度达0.195 ng/mL。叶酸作为水溶性B族维生素，在核酸合成、氨基酸代谢及细胞增殖中发挥关键作用，其水平异常与贫血、神经管畸形等疾病密切相关，小鼠模型常被用于叶酸代谢相关机制研究。本试剂盒包含预包被捕获抗体、生物素标记检测抗体、标准品及显色底物，通过优化反应体系实现高特异性检测，适用于科研场景下的小鼠营养学研究、叶酸缺乏模型评估、代谢性疾病干预实验等，可精准追踪体内叶酸动态变化，为探索叶酸在生长发育、氧化应激或免疫调节中的功能提供可靠工具。实验操作简便，3.5小时内即可完成检测，配套标准曲线和详细操作指南确保数据重复性。
别名：

N/A
缩写：

FA
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

0.195 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-105
1:2	Average %	89
	Range %	83-96
1:4	Average %	92
	Range %	84-99
1:8	Average %	91
	Range %	85-99

回收率：

The recovery of mouse FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-100
EDTA plasma (n=4)	95	89-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究进展：

叶酸（folic acid），也称为维生素B9，是人体必需的水溶性维生素之一。最新研究表明，叶酸不仅对于预防妊娠期神经管缺陷有重要作用，还可能有助于预防其他出生缺陷，如唇裂、腭裂、心脏病等。此外，一些研究还发现，叶酸摄入量不足可能与阿尔茨海默病、帕金森病等神经系统疾病的发生和发展有关。因此，保证足够的叶酸摄入量对于维护人体健康具有重要意义。