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Human Folic acid(FA) ELISA Kit

  • 中文名称:
    人叶酸(FA)酶联免疫试剂盒
  • 货号:
    CSB-E17109h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO人叶酸(FA)酶联免疫检测试剂盒(货号CSB-E17109h),基于竞争法定量原理设计,适用于科研实验中血清或血浆样本的叶酸水平检测。叶酸作为水溶性B族维生素,在细胞增殖、DNA合成及氨基酸代谢中发挥重要作用,其水平异常与巨幼红细胞性贫血、神经管畸形等病理过程相关。试剂盒检测灵敏度达12.5 pg/mL,线性范围为12.5-200 pg/mL,采用预包被96孔板搭配即用型标准品与检测试剂,实验过程包含样本预处理、孵育反应及酶标仪读数等步骤,可在4.5小时内完成检测。该试剂盒适用于营养学研究、代谢性疾病机制探索、药物干预效果评估等科研领域,特别在叶酸代谢与妊娠健康、血液系统疾病关联性研究中具有应用价值。配套提供详细操作手册,包含标准曲线构建方法和样本浓度计算公式,实验全程无需复杂设备,适用于常规实验室开展批量样本分析。
  • 别名:
    N/A
  • 缩写:
    FA
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 检测范围:
    12.5 pg/mL-200 pg/mL
  • 灵敏度:
    6.25 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 89  
    Range % 84-93  
    1:40 Average % 93  
    Range % 89-97  
    1:80 Average % 95  
    Range % 90-100  
    1:160 Average % 84  
    Range % 80-88  
  • 回收率:
    The recovery of human FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-105  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.093 0.090 0.092    
    100 0.227 0.208 0.218    
    50 0.378 0.367 0.373    
    25 0.618 0.637 0.628    
    12.5 0.986 1.003 0.995    
    0 1.485 1.429 1.457    
  • 本试剂盒所含材料:
      • A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human FA antibody.
      • Standard (10 x concentrate)(1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • HRP-conjugated FA (100 x concentrate) (1 x 6oμl)
      • HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated FA antibody solution.
      • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
      • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理:
  • 货期:
    3-5 working days

产品评价

相关问答

 常见问题解答
Q:

We are looking for Elisa kit for human folic acid, detection range: 310-1000 nmol/L.
Material: erythrocytes (not full blood)
Please tell me is the result obtained from your kit can be comparable to the microbiological reference method?

A:
Thank you for your enquiry!Currently we just have this kit, and the detailed information, pls check the manual.
The theory of different detection methods are different.Since we have no information which is comparable to the microbiological reference method.
Pls just take your own actual test result.

靶点详情

  • 最新研究进展:
        叶酸(Folic acid)是一种维生素B族成员,参与人体DNA、RNA的合成和修复,对于细胞生长和分裂非常重要。最新的研究表明,叶酸在人体内具有多种生理功能,包括对于心血管健康和神经系统健康的影响,以及对于出生缺陷和某些类型的癌症的预防作用。
        一项对临床试验的回顾性分析发现,叶酸可以降低缺血性心血管疾病(如冠心病和脑卒中)的发生率和死亡率。另一项研究显示,叶酸可以促进神经细胞的发育和修复,有助于预防老年痴呆症和帕金森病等神经系统疾病的发生。
    此外,叶酸还可以预防某些类型的出生缺陷,如脊柱裂和唇裂等,因为这些缺陷通常与母体叶酸不足有关。对于某些类型的癌症,如结肠癌和乳腺癌等,一些研究表明,适当的叶酸摄入可以减少其发生的风险。