Rat Dihydrotestosterone,DHT ELISA Kit

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中文名称：

大鼠双氢睾酮(DHT)酶联免疫试剂盒
货号：

CSB-E07879r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠双氢睾酮（Dihydrotestosterone, DHT）酶联免疫检测试剂盒（货号：CSB-E07879r），采用竞争法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中的DHT含量，检测灵敏度为10 pg/mL，线性检测范围为10 pg/mL至2000 pg/mL。DHT是睾酮经5α-还原酶催化生成的活性雄激素，在调节雄性生殖系统发育、维持第二性征及毛发生长等生理过程中发挥关键作用，其异常水平与激素相关疾病机制研究密切相关。本试剂盒通过特异性抗体捕获样本中的DHT分子，结合酶标物竞争反应实现精准定量，操作流程包括样本预处理、孵育、洗涤和显色等步骤，可在3.5小时内完成检测。科研人员可将其应用于内分泌调控研究、雄性生殖生物学实验、药物开发中激素代谢评估，或探究前列腺疾病、脱发等病理模型的激素水平变化。试剂盒提供预包被板条、标准品、检测抗体及全套实验缓冲液，需自备酶标仪（450nm波长）和移液设备，实验结果需结合标准曲线进行定量分析。本产品仅用于科学研究，不适用于医疗诊断或其他用途。
别名：

N/A
缩写：

DHT
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

10 pg/mL-2000 pg/mL
灵敏度：

5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-106
1:2	Average %	93
	Range %	89-97
1:4	Average %	95
	Range %	89-99
1:8	Average %	102
	Range %	98-106

回收率：

The recovery of rat DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	102	97-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.156	0.160	0.158
500	0.312	0.329	0.321
160	0.498	0.528	0.513
40	0.818	0.787	0.803
10	1.316	1.278	1.297
0	1.960	1.954	1.957

本试剂盒所含材料：
- A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit DHT antibody.
- Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- Anti-mouse DHT Antibody 1 x 6 ml -- Act as the detection antibody.
- HRP-conjugate 1 x 6 ml -- Bind to the DHT antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
- Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
- Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
- Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
- Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days