Canine Dihydrotestosterone,DHT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬双氢睾酮（Dihydrotestosterone, DHT）酶联免疫试剂盒（货号：CSB-E07881c）是一款专为科研设计的检测工具，适用于定量分析犬血清、血浆及组织匀浆样本中的DHT含量。DHT作为睾酮的活性代谢产物，在雄性生殖系统发育、毛发生长及前列腺功能调控中发挥重要作用，其水平检测对研究犬类内分泌调节机制及相关疾病模型具有重要价值。CUSABIO试剂盒采用竞争法检测原理，通过抗原抗体特异性结合反应实现精准定量，检测范围为40-8000 pg/mL，可覆盖生物样本中DHT的常规浓度区间。实验操作兼容常规酶标仪（450 nm波长），支持科研人员高效完成样本检测。该产品适用于动物实验中对激素干预效果评估、生殖健康研究或代谢性疾病模型的DHT动态分析，例如前列腺增生、脱发机制或内分泌紊乱相关研究，为探索犬类生理病理机制提供可靠数据支持。试剂盒严格排除交叉反应干扰，确保检测特异性，所有组分经优化验证，满足科研实验的重复性和准确性要求。
缩写：

DHT
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

40 pg/mL-8000 pg/mL
灵敏度：

20 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-103
1:2	Average %	95
	Range %	91-99
1:4	Average %	102
	Range %	98-104
1:8	Average %	89
	Range %	82-95

回收率：

The recovery of canine DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	99	98-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.