Human Dihydrotestosterone,DHT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人双氢睾酮（Dihydrotestosterone, DHT）酶联免疫检测试剂盒（货号：CSB-E07878h），采用竞争法定量原理，适用于血清、血浆及组织匀浆样本中DHT含量的精准分析。DHT作为睾酮的活性代谢物，在调节男性第二性征发育、前列腺功能及毛发生长等生理过程中发挥关键作用，其水平异常与多种内分泌疾病及代谢研究密切相关。该试剂盒检测线性范围为10 pg/mL至2000 pg/mL，可灵敏捕捉样本中微量DHT变化，为科研人员提供可靠数据支持。实验操作基于抗原-抗体的特异性结合，通过竞争反应实现目标物的定量检测，兼容多种生物样本类型，满足实验室对复杂样本的处理需求。本产品可广泛应用于激素作用机制研究、内分泌系统功能评估、前列腺相关疾病模型构建及药物干预效果分析等科研领域，为探索DHT在生理病理过程中的调控网络提供高效工具，适用于细胞实验、动物模型及临床前研究的数据获取。
别名：

N/A
缩写：

DHT
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

10 pg/mL-2000 pg/mL
灵敏度：

5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DHT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-97
1:2	Average %	89
	Range %	85-99
1:4	Average %	93
	Range %	86-105
1:8	Average %	94
	Range %	87-103

回收率：

The recovery of human DHT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	97	92-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.