Rat Copeptin ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专为大鼠样本设计的高灵敏度和肽素(copeptin)酶联免疫检测试剂盒（货号：CSB-E16335r），适用于血清、血浆及组织匀浆样本的定量分析。和肽素作为血管加压素前体的C端肽段，是反映机体渗透压变化和应激状态的重要生物标志物，在心血管疾病、水电解质平衡及内分泌调控等研究领域具有重要科研价值。试剂盒采用双抗体夹心法原理，检测范围为62.5-4000 pg/mL，具备高特异性与准确性，可灵敏检测微量样本中的目标蛋白。该产品适用于实验室研究中对大鼠模型在病理生理状态下的和肽素水平动态监测，例如心血管疾病机制探索、应激反应评估或肾脏功能相关研究，为深入解析相关疾病的分子机制提供可靠工具。实验操作流程标准化，配套提供完整试剂组分，需自备酶标仪等基础实验设备。本试剂盒仅用于科学研究，不适用于医疗诊断或其他商业用途。
缩写：

copeptin
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	89-102
1:2	Average %	94
	Range %	91-99
1:4	Average %	99
	Range %	96-106
1:8	Average %	97
	Range %	90-102

回收率：

The recovery of rat copeptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-108
EDTA plasma (n=4)	88	82-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.