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Human copeptin ELISA kit

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人和肽素（copeptin）酶联免疫试剂盒（货号：CSB-E12130h）基于夹心法原理设计，适用于血清、尿液、细胞培养上清及组织匀浆样本中copeptin的定量检测，检测范围为78-5000 pg/mL。Copeptin是血管加压素原（preprovasopressin）的C端肽段，与血管加压素共同释放，其稳定性使其成为评估机体应激反应（如心血管疾病、脓毒症等病理状态）的重要生物标志物。CUSABIO试剂盒采用高特异性抗体对，可精准捕获样本中的目标蛋白，结合显色系统实现定量分析，操作流程标准化且重复性良好。适用于心血管疾病机制研究、脓毒症病理模型探索、水盐代谢相关基础实验等领域，支持体外实验中copeptin水平的动态监测及生物标志物相关性分析，为应激相关疾病的基础研究提供高效检测工具。试剂盒兼容常规实验室设备，满足多类型生物样本的检测需求，实验参数经过严格优化，确保数据可靠性。
缩写：

copeptin
种属：

Homo sapiens (Human)
样本类型：

serum, urine, cell culture supernates, tissue homogenates
检测范围：

78 pg/mL-5000 pg/mL
灵敏度：

19.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-95
1:2	Average %	99
	Range %	92-105
1:4	Average %	97
	Range %	90-106
1:8	Average %	96
	Range %	91-102

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.535	2.752	2.644	2.533
2500	2.012	2.179	2.096	1.985
1250	1.598	1.599	1.599	1.488
625	0.847	0.867	0.857	0.746
312	0.478	0.502	0.490	0.379
156	0.336	0.356	0.346	0.235
78	0.237	0.221	0.229	0.118
0	0.109	0.112	0.111

本试剂盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Human copeptin antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled copeptin antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.