Mouse copeptin ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠和肽素（copeptin）酶联免疫检测试剂盒（货号CSB-E14921m），采用双抗体夹心法原理实现定量分析，可精准检测血清、血浆及组织匀浆样本中的靶蛋白含量。和肽素作为精氨酸加压素（AVP）的C端片段，是反映神经内分泌应激反应的重要生物标志物，在调节体液平衡、心血管功能及应激反应研究中具有重要价值。本试剂盒检测范围为3.12-200 pg/mL，灵敏度高，操作流程包含标准品梯度稀释、样本孵育、酶标抗体结合及显色反应等步骤，实验全程约需4.5小时。试剂盒提供预包被捕获抗体的96孔板、系列浓度标准品、检测抗体及终止液等核心组分，配套实验方案优化了孵育时间和反应条件，确保数据稳定可靠。适用于基础科研中探讨小鼠模型在应激性疾病、水盐代谢异常、心血管功能障碍或神经内分泌紊乱等生理病理过程中的分子机制，为动物实验提供高效的分析工具。所有组分需在2-8℃保存，开封后标准品建议分装冻存以延长稳定性。
缩写：

copeptin
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	96
	Range %	92-102
1:4	Average %	102
	Range %	96-108
1:8	Average %	94
	Range %	89-99

回收率：

The recovery of mouse copeptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-98
EDTA plasma (n=4)	88	82-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.