Rat Complement fragment 3a,C3a ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠补体片断3a（C3a）酶联免疫检测试剂盒（货号：CSB-E08510r），基于双抗体夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本中C3a蛋白的精准分析。C3a是补体系统活化过程中产生的关键炎症介质，参与调节过敏反应、趋化作用及免疫应答，其水平变化与炎症性疾病、感染及组织损伤等病理过程密切相关。本试剂盒检测灵敏度覆盖1.56 ng/mL至100 ng/mL宽动态范围，可稳定识别样本中痕量目标蛋白。通过优化的预包被抗体与酶标记检测抗体协同作用，确保检测结果的高特异性和重复性，适用于科研场景中炎症机制研究、免疫相关疾病动物模型评估或药物干预实验中C3a动态变化的监测，为探索补体系统在生理病理中的作用提供可靠工具。实验操作简便，兼容常规实验室设备，配套标准品及检测缓冲液，支持快速完成样本检测与数据分析。
缩写：

C3a
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	88
	Range %	80-97
1:10	Average %	95
	Range %	92-102
1:20	Average %	96
	Range %	90-102
1:40	Average %	93
	Range %	90-96

回收率：

The recovery of rat C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	101	95-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.