Rabbit Complement fragment 3a,C3a ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于科研领域的兔子补体片段3a（C3a）酶联免疫检测试剂盒（货号：CSB-E09283Rb），可定量分析血清、血浆及组织匀浆样本中的C3a蛋白水平。C3a是补体系统活化过程中产生的关键炎症介质，参与调节免疫反应、趋化炎症细胞及促进组织修复等生物学过程，在炎症性疾病和免疫调控研究中具有重要价值。试剂盒采用双抗体夹心法原理，通过预包被特异性抗体实现目标蛋白的高效捕获，检测灵敏度可达6.25 ng/mL，线性范围为6.25-400 ng/mL，能够稳定检测不同浓度梯度的样本。该试剂盒适用于兔源实验模型（如败血症、自身免疫疾病或组织损伤模型）中补体系统激活程度的评估，可帮助研究者分析炎症反应机制、药物干预效果或病理状态下C3a的动态变化。实验操作流程标准化，提供完整的检测组分，包括预包被酶标板、标准品、检测抗体及显色系统，需自备酶标仪进行OD值测定。本产品严格适用于实验室研究，不涉及其他用途。
缩写：

C3a
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 ng/mL-400 ng/mL
灵敏度：

1.56 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	80-100
1:2	Average %	96
	Range %	85-104
1:4	Average %	97
	Range %	92-104
1:8	Average %	90
	Range %	82-98

回收率：

The recovery of rabbit C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	84-107
EDTA plasma (n=4)	96	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.