Mouse Complement fragment 3a,C3a ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠补体片段3a（C3a）酶联免疫试剂盒（CSB-E08511m）采用双抗体夹心法原理，专为定量检测血清、血浆、细胞培养上清及组织匀浆样本中的C3a蛋白设计。C3a是补体系统活化过程中产生的关键过敏毒素，通过与特定受体结合介导炎症反应、免疫细胞趋化及组织修复等生理病理过程，在免疫调控和炎症性疾病研究中具有重要价值。本试剂盒检测灵敏度为0.156 ng/mL，线性范围覆盖0.156-10 ng/mL，可精准检测低丰度样本。实验采用预包被抗体的96孔板和生物素标记检测抗体，通过链霉亲和素-HRP催化显色反应实现靶标定量，操作流程包含样本孵育、洗涤、显色及终止等标准化步骤。该产品适用于基础免疫学研究中补体系统激活机制的探索、炎症性疾病动物模型的生物标志物分析，以及药物研发中抗炎或免疫调节剂的效果评估。实验前需注意避免样本反复冻融，血浆样本建议使用EDTA或肝素抗凝剂，组织匀浆需离心去除沉淀以确保检测准确性。
缩写：

C3a
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	93
	Range %	89-100
1:20	Average %	96
	Range %	91-101
1:40	Average %	99
	Range %	93-105
1:80	Average %	88
	Range %	82-95

回收率：

The recovery of mouse C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-104
EDTA plasma (n=4)	93	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.